CNBr-activated Bestarose 4B is a pre-activated agarose resin with cyanogen bromide. Biomolecules with a primary amino group (-NH2) can be covalently coupled to this resin for making specific affinity resin and immobilized enzymes.
The resin has the following characteristics:
Mild reaction conditions with protein and other biological macromolecules.
Can be directly coupled to biological macromolecules without coupling spacers.
The ligand and the scaffold can form a multi-site coupling, the ligand should not fall off.
Applied to the coupling of proteins, peptides, nucleic acids, etc.
Appearance |
White slurry, can be layered /white powder solid |
Matrix |
Cross-linked 4% agarose |
Particle size+ |
45~165μm |
Conjugated functional groups |
Primary amino group |
Amount of conjugated protein |
25-60mg α-chymotrypsinogen/mL resin |
pH stability++ |
3~11(working) 2~11(CIP) |
Chemical stability+++ |
Stable in common aqueous buffers: 6M GuHCl,8M Urea, 70% ethanol |
Max. flow velocity |
≥15cm/h(BXK26/40,H=30cm, 20℃,in water) |
Max. pressure |
0.16bar |
Storage |
2 ~ 8℃, 100% acetone |
+ The particle size is normally distributed, and the particles in this range account for more than 95% of the total.
++ No coupling ligand .
+++ Data when the coupled ligand is also stable.
Product |
Cat. No. |
Pack size |
CNBr-activated Bestarose 4B |
AA017305 |
25mL |
AA017307 |
100mL |
|
AA0012 |
500mL |