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CNBr-activated Bestarose 4B

CNBr-activated Bestarose 4B

Product Characteristics

  • Mild reaction conditions with protein and other biological macromoleculesCan be directly coupled to biological macromolecules without coupling spacersThe ligand and the scaffold can form a multi-site coupling, the ligand should not fall off
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1. Introduction

CNBr-activated Bestarose 4B is a pre-activated agarose resin with cyanogen bromide. Biomolecules with a primary amino group (-NH2) can be covalently coupled to this resin for making specific affinity resin and immobilized enzymes.

The resin has the following characteristics:

         Mild reaction conditions with protein and other biological macromolecules.

         Can be directly coupled to biological macromolecules without coupling spacers.

         The ligand and the scaffold can form a multi-site coupling, the ligand should not fall off.

         Applied to the coupling of proteins, peptides, nucleic acids, etc.

2. Technical characteristics

Appearance

White slurry, can be layered /white powder solid

Matrix

Cross-linked 4% agarose

Particle size+

45~165μm

Conjugated functional groups

Primary amino group

Amount of conjugated protein

25-60mg α-chymotrypsinogen/mL resin

pH stability++

3~11(working) 2~11(CIP)

Chemical stability+++

Stable in common aqueous buffers: 6M GuHCl,8M Urea, 70% ethanol

Max. flow velocity

≥15cm/h(BXK26/40,H=30cm, 20℃,in water)

Max. pressure

0.16bar

Storage

2 ~ 8℃, 100% acetone

+ The particle size is normally distributed, and the particles in this range account for more than 95% of the total.

++ No coupling ligand .

+++ Data when the coupled ligand is also stable.

 

3. Order information

Product

Cat. No.

Pack size

CNBr-activated Bestarose 4B

AA017305

25mL

AA017307

100mL

AA0012

500mL

 

 

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