Affinity chromatography resin is an important material specifically used in the affinity chromatography process. This resin material is used to purify and separate specific biomolecules (such as proteins) based on their affinity or specific interactions with specific ligands or molecules.
The resins used in affinity chromatography typically consist of a solid support matrix, often made of a material such as agarose or other polymers, to which ligands with specific affinity for the target molecule are attached. Ligands can be antibodies, enzymes, or other molecules with high affinity for the target biomolecule.
Physical properties of affinity chromatography resins
Crosslinked 4%
beaded agarose
Crosslinked 6%
beaded agarose
Superflow agarose (highly crosslinked)
UltraLink Biosupport
Bead size range
45 to 165 µm
45 to 165 µm
45 to 165 µm
50 to 80 µm
Exclusion limit
20,000 kDa
4000 kDa
6000 kDa
2000 kDa
Pressure limit
0.35 MPa
0.35 MPa
0.65 MPa
0.69 MPa
Methods
gravity-flow or low-speed centrifugation
gravity-flow or low-speed centrifugation
FPLC systems,
gravity-flow
FPLC systems, HPLC, gravity-flow
Coupling capacity
medium
medium
medium
high
pH range
3–11
3–11
2–12
1–13
What are the different types of affinity chromatography resins?
There are various types of affinity chromatography resins designed to cater to different biological molecules and their specific interactions. Here are some common types:
1. Antibody purification
Antibody purification resins rely on highly specific affinity between antibody and antigen to achieve the separation purpose.
2. Tagged Protein Purification
Tagged proteins are fusion proteins obtained by combining the N site or C site of proteins with proteins or peptide tags (with specific adsorption) from substances (for example, metal ions or dextran) via fusion expression.
3. Race-based affinity resin
Race-based affinity resins can specifically bind with substances with certain structure or similar function. Despite its relative weak specificity, race-based affinity resin can achieve chromatography method with high resolution by choosing suitable binding and elution conditions.
4. Plasmid purification resin
Since the exposure level of charge and basic groups differs on the DNA surface of open circular plasmid and closed-loop supercoiled plasmid, plasmid purification resin can achieve the purification of closed-loop supercoiled plasmids via sulfophilic adsorption of 2-Pyridine (i.e. hydrophilic-hydrophobic mixed mode).
5. Pre-activated resin
Pre-activated resins enjoy advantages such as wide application, good chemical and mechanical stability, flexibility in ligands and matrix choice as well as the flexibility in choosing coupling methods according to ligand property.
How does affinity chromatography resin work?
Affinity chromatography resin works by leveraging specific interactions between a target biomolecule and an immobilized ligand on a solid support matrix. The process involves applying a sample to the resin, allowing specific binding of the target, washing away non-specific molecules, and then eluting the purified target from the resin. This technique selectively isolates and purifies biomolecules based on their unique affinities, making it a powerful tool in biochemistry and biotechnology.
What are the main uses of affinity chromatography resins?
Application
Description
Protein Purification
Used to purify proteins by employing resins with ligands specific to the target protein.
Enzyme Purification
Enables the purification of enzymes using resins with ligands that selectively bind to the enzyme of interest.
Antibody Purification
Utilizes ligands on the resin to selectively bind and isolate monoclonal antibodies and other antibody molecules.
Nucleic Acid Purification
Involves the use of complementary ligands on the resin to target specific DNA or RNA sequences for purification.
Cell Separation
Applied in cell biology to isolate specific cell types based on interactions with surface markers on the resin.
Drug Discovery and Development
Used for the purification of drug targets and candidates, aiding in the identification and isolation of potential therapeutics.
Biotechnology Applications
Employed in various biotechnological processes, including the purification of recombinant proteins and isolation of biomolecules for diagnostics.
Study of Protein-Protein Interactions
Facilitates the investigation of protein-protein interactions by immobilizing one protein on the resin to study its binding partners.
Virus Purification
Utilized in the purification of viruses by designing ligands on the resin to interact specifically with viral particles.
What kind of affinity chromatography resin is good?
✔ Specificity: The resin demonstrates a high level of specificity, selectively binding the target biomolecule with precision while minimizing non-specific interactions.
✔ Capacity: It possesses a substantial binding capacity, ensuring the efficient capture and purification of the target molecule with a high yield.
✔ Selectivity: The resin maintains selectivity, effectively isolating the target molecule even in complex mixtures containing various biomolecules.
✔ Stability: Chemically and mechanically stable under chromatographic conditions, the resin ensures long-term performance and reusability.
✔ Reusability: Capable of regeneration, the resin allows for multiple uses without a significant loss of binding capacity.
✔ Low Ligand Leaching: The resin releases minimal amounts of ligands into the purified sample, preventing contamination and preserving the purity of the eluted biomolecule.
Who are the major suppliers of affinity chromatography resins?
● GE Healthcare (Cytiva): A leading life sciences company, GE Healthcare, now known as Cytiva, offers a comprehensive range of affinity chromatography resins. Renowned for the HiTrap and Sepharose product lines, they provide solutions for protein purification and separation.
● Merck Millipore: Merck Millipore, part of Merck Group, is a global supplier of life science and analytical products. Their chromatography resins, including HisTrap and Ni Sepharose, are widely used for purifying proteins and other biomolecules.
● Bio-Rad: Bio-Rad is a multinational manufacturer and distributor of life science research and clinical diagnostic products. Their chromatography resin offerings, such as Profinity and CHT Ceramic Hydroxyapatite, cater to diverse purification needs.
● Thermo Fisher Scientific: Thermo Fisher Scientific is a major player in the scientific research and healthcare industry. Their chromatography resins, including Pierce and HisPur, are well-regarded for applications in protein purification and analysis.
● Agilent Technologies: Agilent Technologies is a global leader in analytical instrumentation and life sciences solutions. Their chromatography resins, like HisTrap FF, contribute to efficient protein purification processes.
● Pall Corporation (Danaher): Acquired by Danaher Corporation, Pall Corporation specializes in filtration, separation, and purification technologies. Their chromatography resins, such as His60 Ni Superflow Resin, are trusted for diverse bioseparation applications.
● Tosoh Bioscience: Tosoh Bioscience is a Japanese company known for its chromatography and separation technologies. Their chromatography resins, including Toyopearl and TSKgel, are utilized in research, bioprocessing, and pharmaceutical applications.
● Repligen: Repligen Corporation focuses on developing and commercializing bioprocessing technologies. Their chromatography resins, like CaptivA PriMAB, address the purification needs of biopharmaceutical manufacturing.
FAQs about affinity chromatography resins
1. Can affinity chromatography resins be regenerated for reuse?
Answer: Many affinity chromatography resins can be regenerated to allow for multiple uses without a significant loss of binding capacity. Manufacturers typically provide guidelines for proper regeneration procedures.
2. Are there any considerations for the storage of affinity chromatography resins?
Answer: Affinity chromatography resins should be stored according to the manufacturer's recommendations. Proper storage conditions, such as temperature and avoiding exposure to contaminants, are crucial to maintaining resin integrity.
3. What types of biomolecules can be purified using affinity chromatography resins?
Answer: Affinity chromatography resins can be tailored to purify various biomolecules, including proteins, enzymes, antibodies, nucleic acids, and other molecules, depending on the specificity of the ligands immobilized on the resin.
4. How do I choose the right affinity chromatography resin for my application?
Answer: The choice of resin depends on factors such as the target biomolecule, ligand specificity, binding capacity, and the intended application. Consideration of these factors, along with the manufacturer's guidelines, can guide the selection process.
5. What are some common ligands used in affinity chromatography resins?
Answer: Ligands vary based on the target biomolecule. Common ligands include antibodies (Protein A/G), nickel (Ni-NTA) for histidine-tagged proteins, glutathione for GST-tagged proteins, and others tailored to specific interactions.
Conclusion
Affinity resins are solid support matrices used in biopharmaceutical and biotechnology laboratories for the purification of a range of biomolecules. If you need to purchase large quantities of affinity resin materials, Bestchrom can provide you with relevant services and assistance.
As one of China's leading chromatography resin manufacturers and exporters, Bestchrom is fully capable of providing high-quality resin materials for your projects. By choosing Bestchrom's products and services, you can save money, optimize your laboratory budget, and maximize your Assigned to other key areas.
If you are interested in our affinity resin products, please visit our website or contact us by email.
Established in 2008, Bestchrom Biosciences Ltd. is a professional manufacturer focusing on providing high-quality chromatography resins and resin-based services and solutions.
We are a leader in resin-based separation of China with the capability to produce chromatography resins on bio-process scale. Bestchrom offers a wide range of separation resins, pre-packed columns, lab and process scales empty columns, microcarriers, and customization services.
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