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Mixed-mode chromatography is an innovative chromatography method which can simultaneously provide different interactions for the binding between ligands and macro-biomolecules. Dominant interactions in this category include electric charge interaction, hydrophobic interaction and hydrogen bond. Compared with single-mode methods, mixed-mode chromatography can achieve elution by changing pH, conductivity or adopting pH-salt double gradient strategy, which expands application conditions, providing effective solutions to challenges faced in processing and purification practice.
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