AT Protein A Diamond

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1. Introduction

AT Protein A Diamond is A new type of affinity chromatography resin made by coupling alkali-resistant Protein A with high rigid agarose substrate through epoxy activation. Compared with traditional antibody affinity resin, the ligand is more stable, the flow velocity is faster, and the back pressure is lower. Suitable for capturing monoclonal antibodies or Fc fusion proteins from mass cell culture media and polyclonal antibodies from ascites or plasma.

The ligand of AT Protein A Diamond is the fragment of recombinant Protein A obtained by fermentation of E.Coli.During upstream construction, alkali-resistant amino acids were used to replace the non-alkali-resistant amino acids, and protease-sensitive amino acids were removed, so that the ligand has good alkali-stability. The ligand fermentation and subsequent purification processes use no animal raw materials. The resin can be cleaned in situ with 0.1~0.5M NaOH, avoiding the use of expensive and corrosive cleaning reagents, which can effectively save costs, and is easy to be used in industrial large-scale production.

2. Technical Characteristics

Appearance	White slurry, which can be layered
Matrix	High rigidity agarose
Particle size⁺	40~120μm
Functional group	Recombinant Alkali Tolerant Protein A (24KD)
Cross-linking method	Epoxy chemistry
Dynamic binding capacity⁺⁺	≥40mg human IgG/mL packed resin
Chemical stability	Stable in common aqueous buffers: 10mM HAc, 0.1M sodium citrate，6M GuHCl,8M Urea, 30%isopropyl alcohol, 20% ethanol.
Pressure flow velocity	~500cm/h，BXK300，H=20cm，
Max. pressure	0.5MPa
pH stability	3~12(working) 2~13(CIP) CIP: 0.1~0.5M NaOH
Storage⁺⁺⁺	2~8℃, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	100~300cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.

++Linear flow velocity 100cm / h, column height 10cm, buffer conditions: 20mM PB, 0.15M NaCl, pH7.4

+++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
AT Protein A Diamond	AA0272	25mL
	AA0273	100mL
	AA0274	500mL
	AA0275	1L
	AA0276	5L
	AA0277	10L
	AA302315	20L

Prepacked columns	Code No.	Pack size
EzFast AT Protein A Diamond	EA302301	1×1mL
	EA302303	1×5mL
	EA302351	5×1mL
	EA302353	5×5mL
EzScreen AT Protein A Diamond	EA02725	1×4.9mL
EzScreen AT Protein A Diamond	EA02735	5×4.9mL
EzLoad 16/10 AT Protein A Diamond	EA302304	1 pcs
EzLoad 26/10 AT Protein A Diamond	EA302306	1 pcs

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Protein A Affinity Resins