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Protein A Affinity Resins

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Protein A Affinity Resins

Extrem A Diamond

Product Characteristics

  • Under the same contact time, it has a higher dynamic binding capacity than conventional products.
  • The high alkali tolerance of protein ligands can withstand CIP of 0.5~1.0 M NaOH during the cleaning process.
  • High binding capacity, can reduce production costs and improve production efficiency.
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Description
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1. Introduction 

Extrem A Diamond is a new generation of enhanced alkali-resistant resin independently developed by Bestchrom.The protein A ligand is expressed in E. coli.Compared with previous AT Protein A Diamond Plus, it enjoys higher tolerance towards alkali (can tolerate CIP with 0.5-1.0M NaOH) and higher binding capacity. They can effectively cut cost during scale-up by choosing lower column volume. Besides, they are suitable for capturing monoclonal antibodies or Fc fusion protein from large volume cell culture media, as well as the capture of polyclonal antibodies from ascites or plasma.
The resin has the following characteristics：
Under the same contact time, it has higher dynamic binding capacity than conventional products.
The high alkali tolerance of protein ligands can withstand CIP of 0.5~1.0 M NaOH during the cleaning process.
High binding capacity, can reduce production cost and improve production efficiency.

2. Technical Characteristics

Appearance

White slurry, which can be layered

Matrix

High rigidity agarose

Average particle size +

~60μm

Functional group

Recombinant Alkali Tolerant Protein A

Cross-linking method

Epoxy chemistry

Dynamic binding capacity ++

≥75mg Human IgG/mL packed resin(Residence time 6min)

Chemical stability

Stable in common aqueous buffers: 10mM HCl, 0.1Msodium citrate,6M GuHCl,8M Urea, 30% Isopropyl alcohol, 20% ethanol.

Max. pressure

0.5MPa

Pressure flow velocity

≥300cm/h,

pH stability

3~12(working) 2~14(CIP)

Storage+++

2~8℃, 20% ethanol or 2% benzyl alcohol
 
+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++Linear flow velocity 100cm / h, column height 10cm, buffer conditions: 20mM PB, 0.15M NaCl, pH7.4
+++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product

Code No.

Pack size

Extrem A Diamond

AA04501

25mL

AA04502

100mL

AA04503

500mL

AA04504

1L

AA04505

5L

AA04506

10L
 

Prepacked columns

Code No.

Pack size

EzFast Extrem A Diamond

EA04521

1×1mL

EA04523

1×5mL

EA04531

5×1mL

EA04533

5×5mL

EzScreen Extrem A Diamond

EA04525

1×4.9mL

EA04535

5×4.9mL

EzLoad 16/10 Extrem A Diamond

EA04501

1 pcs

EzLoad 26/10 Extrem A Diamond

EA04511

1 pcs
 

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