Benzamidine Bestarose 6B

Product Characteristics

The benzamidines are broad-spectrum inhibitors of serine proteases (including trypsin, thrombin, urokinase, kallikrein, prokinin releasing enzyme, etc) and can be used as ligands for the purification of such substances.

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1. Introduction

Benzamidine Bestarose 6B is an affinity chromatography resin made by coupling p-amino benzamidine to Bestarose 6B agarose gel. It is commonly used for the separation and purification of serine proteases or the removal of serine proteases from biological samples. The benzamidines are broad-spectrum inhibitors of serine proteases (including trypsin, thrombin, urokinase, kallikrein, prokinin releasing enzyme, etc) and can be used as ligands for the purification of such substances.

2. Technical Characteristics

Appearance	White slurry, can be layered
Matrix	Cross-linked agarose, 6%
Particle size⁺	45~165μm
Functional group	P-aminobenzidine
Ligand concentration	~7μmol P-aminobenzidine /mL resin
Dynamic binding capacity++	≥10mg Trypsin/mL packed resin
Operation flow velocity	~75cm/h（BXK16/10，H=5cm，25°C）
Chemical stability	Stable in common aqueous buffers: 6M GuHCl,8M Urea, PH =1, 2, 3, 4 hydrochloric acid solution, 0.025M borax solution with pH=8, 9, 10, 11
pH stability	3~11(working)、2~13(CIP)
Storage+++	2~8℃, 20% ethanol(pH7.0) or 2% benzyl alcohol(pH7.0)

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++Binding capacity is tested at 50mM Tris + 0.5M NaCl pH8.0

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
Benzamidine Bestarose 6B	AA511305	25mL
	AA511307	100mL
	AA511311	500mL
	AA511312	1L
	AA511313	5L
	AA511314	10L

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