Butyl Bestarose HP

Product Characteristics

The framework of Butyl Bestarose HP hydrophobic resin is highly cross-linked agarose, which retains the excellent hydrophilicity and pore structure of natural polysaccharide compoundsand has good compatibility with biological macromolecules.
The average particle size of Butyl Bestarose HP is 34μm, which has a higher resolution efficiency than Butyl Bestarose FF.
The surface contains hydrophobic ligands, the ligand is butyl, which is particularly suitable for the fine separation and purification of recombinant proteins, antibodies, vaccines, VLP particles and other highly hydrophobic biological molecules.

Butyl-S Bestarose FF

Diamond Butyl Mustang

Description

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1. Introduction

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

The framework of Butyl Bestarose HP hydrophobic resin is highly cross-linked agarose, which retains the excellent hydrophilicity and pore structure of natural polysaccharide compoundsand has good compatibility with biological macromolecules. The average particle size of Butyl Bestarose HP is 34μm, which has a higher resolution efficiency than Butyl Bestarose FF. The surface contains hydrophobic ligands, the ligand is butyl, which is particularly suitable for the fine separation and purification of recombinant proteins, antibodies, vaccines, VLP particles and other highly hydrophobic biological molecules.

2. Technical Characteristics

Appearance	White slurry, can be layered
Matrix	Highly cross-linked agarose, 6%
Functional group	Butyl
Ligand concentration	~50μmol Ligand/mL resin
Dynamic binding capacity	38mg β-Lactoglobulin/mL packed resin
Particle size⁺	24~44μm(the average particle size is 34μm)
Max. pressure	0.3 MPa
Pressure flow velocity	≥200cm/h(0.1MPa BXK50/30 H=15cm 25℃)
Chemical stability	Stable in common aqueous buffers: 1M NaOH++、1M HAc++、6M GuHCl、30% isopropyl alcohol、70% ethanol
pH stability	2~14(CIP),3~12(working)
Temperature tolerance	Working temperature: 2~40℃, Can't freeze, Can tolerate 121℃ high pressure sterilization (20min)
Storage+++	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	60~120cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.

++1M NaOH and 1M HAc only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
Butyl Bestarose HP	AH0071	25mL
	AH203107	100mL
	AH203111	500mL
	AH0073	1L
	AH0074	5L
	AH203114	10L
	AH203115	20L
	AH203116	40L

Prepacked columns	Code No.	Pack size
EzFast Butyl HP	EH00721	1×1mL
	EH103151	5×1mL
	EH103103	1×5mL
	EH103153	5×5mL
EzScreen Butyl HP	EH00725	1×4.9mL
EzScreen Butyl HP	EH00735	5×4.9mL
EzLoad 16/10 Butyl HP	EH00701	1 pcs
EzLoad 26/10 Butyl HP	EH00711	1 pcs

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Butyl HIC Resins