Diamond MMC

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1. Introduction

Ion exchange chromatography(IEC)is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields. However, when doing ion exchange chromatography, it is required that the salt content in the sample should not be too high. By introducing benzene ring into the ligand, the compound ion exchange resin has a certain tolerance to salt, which avoids the desalting step of high-salt samples and expands the application range of ion exchange.

IEC resin is composed of three parts: (1)Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability; The Diamond substrate is a highly rigid agarose substrate, which is formed by chemical modification and modification of the traditional Bestarose 6FF substrate with better mechanical properties. (2)Functional group fixed on the substrate, which is a compound charged group, usually consisting of a charged part and a hydrophobic part; (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

Diamond MMC is a multimodal weak cation exchange resin formed by coupling compound groups with benzene ring and carboxyl group on highly rigid agarose microspheres.

2. Technical Characteristics

Appearance	White slurry, can be layered
Matrix	High rigidity agarose
Functional group	Complex weak cationic group
Average particle size+	75μm
Ionic capacity	70~90μmol H⁺/mL packed resin
Dynamic binding capacity ++	> 28 mg BSA/mL packed resin (30mS/cm)
Max. pressure	0.5 MPa
Pressure flow velocity+++	≥1200cm/h（0.5MPa BXK 100/500 , H=20cm,20℃）
Chemical Stability	Stable in common aqueous buffers: 1M NaOH++++,1M HAC++++,6M GuHCl,8M Urea, 70% ethanol ,30% isopropyl alcohol, 20% ethanol, 2% benzyl alcohol Avoid contact with oxidizing agents, cationic detergents.
pH stability	3~14(CIP),3~12(working)
Temperature tolerance	Working temperature:2~40℃, Can't freeze.
Storage+++++	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommend flow velocity	90-500cm/h

+ Average particle size is the accumulated resin particle size of packing volume distribution

++ BXH5/100 column with column height of 10cm, 10%BSA dynamic binding load under the condition of 50mM NaAc+ 0.25m NaCl pH 4.75 and flow velocity of 2mL /min,

+++The flow velocity is a linear flow velocity with BXK100/500 20cm column height at 20℃ and pressure of 0.5MPa

++++1M NaOH and 1M HAc only be used for cleaning

+++++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
Diamond MMC	AI0091	25mL
	AI0092	100mL
	AI305311	500mL
	AI0093	1L
	AI0094	5L
	AI0095	10L
	AI305315	20L
	AI305316	40L

Prepacked columns	Code No.	Pack size
EzFast Diamond MMC	EI00921	1×1mL
	EI315351	5×1mL
	EI315303	1×5mL
	EI315353	5×5mL
EzScreen Diamond MMC	EI00925	1×4.9mL
EzScreen Diamond MMC	EI00935	5×4.9mL
EzLoad 16/10 Diamond MMC	EI044	1 pcs
EzLoad 26/10 Diamond MMC	EI045	1 pcs

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MMC Resins

Diamond MMC

Product Characteristics

1. Introduction

2. Technical Characteristics

3. Order Information

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