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Phenyl HIC Resins

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Hydrophobic Interaction Resins
Phenyl HIC Resins

Diamond Phenyl Mustang

Product Characteristics

  • Diamond Phenyl Mustang is based on fine particle high-rigidity agarose with a hydrophobic phenyl group.
  • The high resolution and low back pressure are suitable for the intermediate purification stage and fine purification stage of protein downstream purification.
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Description
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1. Introduction 

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands. 
Diamond Phenyl Mustang is based on fine particle high-rigidity agarose with a hydrophobic phenyl group.The high resolution and low back pressure are suitable for the intermediate purification stage and fine purification stage of protein downstream purification.

2. Technical Characteristics

Appearance

White slurry, can be layered

Matrix

Highly cross-linked agarose

Functional group

Phenyl

Average particle size

36~44μm

Hydrophobicity

The retention time of Lyzosyme is 45-50min  

Dynamic binding capacity

~19mg BSA/mL packed resin

Max. pressure

0.5MPa

Pressure flow velocity

≥450cm/h(0.5MPa BXK 100/500 H=20cm 20℃)

Chemical stability

Stable in common aqueous buffers: 2M NaOH+、0.5M NaOH+、1M HAc+、 6M GuHCl、8M Urea、30% isopropyl alcohol、30% acetonitrile、2%SDS、3M(NH4)2SO4+ 0.1M KH2PO4(pH7.0)

pH stability

2~14(CIP),3~13(working)

Temperature tolerance

Working temperature: 2~40℃, Can't freeze, Can tolerate 121℃ high pressure sterilization (20min)

Storage++

2~30℃, 20% ethanol or 2% benzyl alcohol

Recommended flow velocity

90~180cm/h

 
+1M NaOH and 1M HAc only be used for cleaning.
++ 2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product

Code No.

Pack size

Diamond Phenyl Mustang

AH301205

25mL

AH301207

100mL

AH301211

500mL

AH301212

1L

AH301213

5L

AH301214

10L

AH301215

20L

AH301216

40L

 

Prepacked columns

Code No.

Pack size

EzFast Phenyl Mustang

EH01421

1×1mL

EH321251

5×1mL

EH321203

1×5mL

EH321253

5×5mL

EzScreen Phenyl Mustang

EH01425

1×4.9mL

EH01435

5×4.9mL

EzLoad 16/10 Phenyl Mustang

EH301204

1 pcs

EzLoad 26/10 Phenyl Mustang

EH301206

1 pcs

 

 

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