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Heparin Bestarose HP

Product Characteristics

  • The resin has the characteristics such as high resolution, physical and chemical stability, firmly attached ligands, long service life, and a wide range of applications.
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1. Introduction 

Heparin Bestarose HP (High Performance) is a family-specific affinity chromatography resin made by covalently coupling heparin to fine particles with high cross-linking agarose. Widely used in the separation and purification of various biological molecules, especially various enzymes, including antithrombin III, thrombin-like, human coagulation factor IX, XI, VIII, lipoprotein lipase, collagenase, DNA polymerase. It can also be combined with human interleukin, human prostate growth factor, recombinant human vascular endothelial growth factor, cartilage growth factor, basic fibroblast growth factor, recombinant human acid fibroblast growth factor, recombinant hepatocyte growth factor, and recombinant murine heparin cofactor II. The combination of recombinant human platelet fourth factor, recombinant human endostatin, recombinant human keratinocyte growth factor and other biological macromolecules.The resin has the characteristics such as high resolution, physical and chemical stability, firmly attached ligands, long service life, and a wide range of applications.

2. Technical Characteristics

Appearance

White slurry,can be layered

Matrix

Highly cross-linked 6% agarose

Particle size+

24~44μm (the average particle size is 34μm)

Functional group

Heparin

Ligand concentration

~10mg Heparin Ligand/mL resin

Dynamic binding capacity

2-5 mg bovine AT III/ mL packed resin

Chemical stability

Stable in common aqueous buffers: 8M urea, 6M GuHCl, 70% ethanol, 50mM sodium acetate(pH4), 10% glycerin, 0.1M NaOH  (20℃, a week)

pH stability

5~10

Storage++

2~30℃, 20% ethanol with 50mM NaAc or 2% benzyl alcohol 50mM NaAc

Recommended flow velocity

<150cm/h

 
+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product

Code No.

Pack size

Heparin Bestarose HP

AA0261

25mL

AA0262

100mL

AA0263

500mL

AA0264

1L

AA0265

5L

AA212114

10L

 

 

Prepacked columns

Code No.

Pack size

EzFast Heparin HP

EA212101

1×1mL

EA050

1×5mL

EA049

5×1mL

EA051

5×5mL

EzScreen Heparin HP

EA02625

1×4.9mL

EA02635

5×4.9mL

EzLoad 16/10 Heparin HP

EA212104

1 pcs

EzLoad 26/10 Heparin HP

EA212106

1 pcs

 

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