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Other Antibody Affinity Resins

IgM Capture Bestarose FF

Product Characteristics

  • This force is strengthened in a high-salt environment and weakened in a low-salt environment.
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Description
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1. Introduction 

IgM Capture Bestarose FF is a sulfur-philic affinity resin made by fixing sulfur-containing compound 2-mercaptopyridine on fine particles of high rigidity agarose. Its optimized ligand density and IgM have suitable affinity, and fine particle microspheres can increase the loading capacity of IgM with larger molecular weight. The principle of sulfur affinity is to use the interaction between the electron donor and the electron acceptor to separate and purify biomolecules. This force is strengthened in a high-salt environment and weakened in a low-salt environment.

Appearance

White slurry, which can be layered

Matrix

High rigidity agarose

Average particle size +

~60μm

Functional group

Recombinant Alkali Tolerant Protein A

Cross-linking method

Epoxy chemistry

Dynamic binding capacity ++

≥75mg Human IgG/mL packed resin(Residence time 6min)

Chemical stability

Stable in common aqueous buffers: 10mM HCl, 0.1Msodium citrate,6M GuHCl,8M Urea, 30% Isopropyl alcohol, 20% ethanol.

Max. pressure

0.5MPa

Pressure flow velocity

≥300cm/h,

pH stability

3~12(working) 2~14(CIP)

Storage+++

2~8℃, 20% ethanol or 2% benzyl alcohol

 

2. Technical Characteristics

Appearance

White slurry, can be layered

Matrix

Highly cross-linked agarose, 6%

Particle size+

45~165μm

Functional group

2-mercaptopyridine

Ligand concentration

~ 2mg 2-mercaptopyridine /mL resin

Chemical stability

Stable in common aqueous buffers:70% ethanol, 30% isopropyl alcohol, 1M HAc++, 0.1M NaOH

Pressure flow velocity

250~400cm/h(0.1MPa BXK50/30 H=25cm 20℃)

Max. pressure

0.3MPa

pH stability

3~11(working),2~13(CIP)

Storage+++

2~30℃,20% ethanol or 2% benzyl alcohol

 
+ The particle size is normally distributed, and the particles in this range account for more than 95% of the total
++ 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product

Code No.

Pack size

IgM Capture Bestarose FF

AA0172

25mL

AA0173

100mL

AA0174

500mL

AA0175

1L

AA0176

5L

AA0177

10L

 

Prepacked columns

Code No.

Pack size

EzFast IgM FF

EA214301

1×1mL

EA042

5×1mL

EH214303

1×5mL

EA043

5×5mL

EzScreen IgM FF

EA01725

1×4.9mL

EA01735

5×4.9mL

EzLoad 16/10 IgM FF

EA214304

1 pcs

EzLoad 26/10 IgM FF

EA214306

1 pcs

 

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