Ni Bestarose HP

Product Characteristics

High resolution, large adsorption capacity, good selectivity, easy regeneration, and low cost.

IMAC Bestarose HP

Chelating Bestarose FF

Description

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1. Introduction

Ni Bestarose HP(High Performance)metal chelation chromatography resin is an affinity chromatography resin made by pre-chelating metal ion Ni2+ on a high-resolution agarose gel with NTA as ligand. It has the advantages of high resolution, large adsorption capacity, good selectivity, easy regeneration, and low cost. It is widely used in the separation and purification of downstream proteins and peptides in biopharmaceutical and bioengineering, especially the efficient purification of histidine-tagged proteins.

2. Technical Characteristics

Appearance	Turquoise slurry, can be layered
Matrix	Cross-linked agarose, 6%
Particle size⁺	24~44μm(the average particle size is 34μm)
Ligand concentration	~15μmol Ligand/mL resin
Dynamic binding capacity	~ 40mg His tag protein/mL packed resin
Chemical stability ⁺⁺	1 week at 40℃: 10mM HCl, 0.1M NaOH, 8M urea, 6M GuHCl; 40℃ 12h: 1M NaOH, 70% acetic acid
Max. pressure	0.3MPa
pH stability⁺⁺⁺	3~12(working) 2~14(CIP)
Storage⁺⁺⁺⁺	2~30℃,20% ethanol or 2% benzyl alcohol
Recommended flow velocity	＜150cm/h

+Particle size is the cumulative resin particle size of packing volume distribution

++ Stability when removing metal ions

+++ CIP refers to pH stability when metal ions are removed.

++++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
Ni Bestarose HP	AA0041	25mL
	AA0042	100mL
	AA0043	500mL
	AA0044	1L
	AA0045	5L
	AA0046	10L
	AA208115	20L

Prepacked columns	Code No.	Pack size
EzFast Ni HP	EA208101	1×1mL
	EA208103	1×5mL
	EA004	5×1mL
	EA005	5×5mL
EzScreen Ni HP	EA00425	1×4.9mL
EzScreen Ni HP	EA00435	5×4.9mL
EzLoad 16/10 Ni HP	EA208104	1 pcs
EzLoad 26/10 Ni HP	EA208106	1 pcs
EzLoad 26/20 Ni HP(Double adaptor）	EA00414	1 pcs

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