Q Bestarose HP

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1. Introduction

Ion exchange chromatography(IEC)is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

IEC resin is composed of three parts: (1)Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability; (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEC resin; (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

Q Bestarose HP(high performance) is a strong anion exchange resin formed by a quaternary amine group on the diamond matrix.

2. Technical Characteristics

Appearance	White slurry, can be layered
Matrix	Highly cross-linked agarose, 6%
Functional group	Quaternary amine group
Particle size+	24~44μm (the average particle size is 34μm)
Ionic capacity	140~200μmol Cl^-/mL packed resin
Dynamic binding capacity ++	~ 75 mg BSA/mL resin
Max. pressure	0.3 MPa
Chemical Stability	Stable in common aqueous buffers: 0.5M NaOH+++,2M NaOH+++,0.1M HCl, 70% formic acid,6M GuHCl,8M Urea, 30% acetonitrile, 30% isopropyl alcohol, 24% ethanol, 70% ethanol, 50mM PB+2M NaCl pH7.0. Avoid contact with oxidizing agents, cationic detergents.
pH stability	2~14 (CIP),2~12(Working)
Temperature tolerance	Working temperature:2~40℃, Can't freeze, Can tolerate 121℃ high pressure sterilization (20min)
Storage++++	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommend flow velocity	60~150cm/h

+ Average particle size is the accumulated resin particle size of packing volume distribution

++ In EzScreen 4.4mL column with column height of 10cm, 10%BSA dynamic binding load at flow velocity of 1mL /min at 50mM Tris+1M NaCl pH8

+++NaOH only be used for cleaning

++++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
Q Bestarose HP	AI0071	25mL
	AI0076	100mL
	AI0072	500mL
	AI0073	1L
	AI0074	5L
	AI0075	10L
	AI204115	20L
	AI204116	40L

Prepacked columns	Code No.	Pack size
EzFast Q HP	EI00721	1×1mL
	EI031	5×1mL
	EI204103	1×5mL
	EI032	5×5mL
EzScreen Q HP	EI00725	1×4.9mL
EzScreen Q HP	EI00735	5×4.9mL
EzLoad 16/10 Q HP	EI034	1 pcs
EzLoad 26/10 Q HP	EI035	1 pcs

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