SP Bestarose BB

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1. Introduction

Ion exchange chromatography(IEC)is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

IEC resin is composed of three parts: (1)Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determining the properties of the IEC resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

Big Beads (BB) series resin is agarose base frame with large particles and highly cross-linked. The large particle base can tolerate samples with poor clarity, it is not easy to plug the column, and the flow velocity is fast, which is suitable for processing large samples in a short time.

SP Bestarose BB is a strong cation exchange resin that can capture biological macromolecules directly from the feed solution.Its high load capacity and fast flow velocity make it suitable for large-scale production applications.

2. Technical Characteristics

Appearance	White slurry, can be layered
Matrix	Cross-linked agarose, 6%
Functional group	Sulfopropyl
Particle size⁺	100~300μm
Ionic capacity	180~250μmol H⁺/mL packed resin
The exclusion limit, Mr	~4× 10⁶ (Globular protein)
Max. pressure	0.3 MPa
Pressure flow velocity⁺⁺	≥1200cm/h（0.1MPa BXK50/30,H=15cm ,20℃,0.1M NaCl）
Chemical stability	Stable in common aqueous buffers: 1M NaOH+++,6M GuHCl,30% isopropyl alcohol,70% ethanol Avoid contact with oxidizing agents
pH stability	3~14(CIP), 4~13 (working)
Temperature tolerance	Working temperature:2~40℃, Can't freeze.
Storage++++	2~30℃, 20% ethanol with 0.2M NaAc or 2% benzyl alcohol with 0.2M NaAc
Recommended flow velocity	200~600cm/h

+Particle size is normally distributed, and particles within this range account for more than 80% of the total.l.

++ The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin

+++1M NaOH only be used for cleaning

++++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
SP Bestarose BB	AI0241	25mL
	AI203407	100mL
	AI0242	500mL
	AI0243	1L
	AI0244	5L
	AI0245	10L
	AI0246	20L
	AI203416	40L

Prepacked columns	Code No.	Pack size
EzFast SP BB	EI02421	1×1mL
	EI203451	5×1mL
	EI203403	1×5mL
	EI203453	5×5mL
EzScreen SP BB	EI02425	1×4.9mL
EzScreen SP BB	EI02435	5×4.9mL
EzLoad 16/10 SP BB	EI203404	1 pcs
EzLoad 26/10 SP BB	EI203406	1 pcs

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