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Benzamidine Bestarose 4FF

Product Characteristics

  • The benzamidines are broad-spectrum inhibitors of serine proteases (such as trypsin, thrombin, urokinase, kallikrein, prokinin releasing enzyme, etc) and can be used as ligands for purifying such substances.
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Description
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1. Introduction 

Benzamidine Bestarose 4FF is an affinity chromatography resin made by coupling p-amino benzamidine to Bestarose 4FF agarose gel. It is commonly used for the separation and purification of serine proteases or the removal of serine proteases from biological samples. The benzamidines are broad-spectrum inhibitors of serine proteases (such as trypsin, thrombin, urokinase, kallikrein, prokinin releasing enzyme, etc) and can be used as ligands for purifying such substances.

2. Technical Characteristics

Appearance

White slurry, can be layered

Matrix

Cross-linked agarose, 4%

Particle size+

45~165μm

Functional group

P-aminobenzidine

Ligand concentration

≥12μmol Ligand/mL resin

Dynamic binding capacity

≥35mg Trypsin/mL packed resin

Max. pressure

0.3 MPa

Pressure flow velocity

>150cm/h(0.1MPa BXK50/30 H=25cm 20℃)

Chemical stability

Stable in common aqueous buffers: 6M GuHCl,8M Urea, PH =1, 2, 3, 4 hydrochloric acid solution, 0.025M borax solution with pH=8, 9, 10, 11

pH stability

2~8(working)、1~9(CIP)

Storage++

2~8℃,20% ethanol with 50mM NaAc(pH4.0) or 2% benzyl alcohol with 50mM NaAc(pH 4.0)

Recommended flow velocity

30-300cm/h

 
+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ 2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product

Code No.

Pack size

Benzamidine Bestarose 4FF

AA0291

25mL

AA0292

100mL

AA111311

500mL

AA0294

1L

AA0295

5L

AA111314

10L

 

Prepacked columns

Code No.

Pack size

EzFast Benzamidine 4FF

EA111301

1×1mL

EA111303

1×5mL

EA111351

5×1mL

EA111353

5×5mL

EzScreen Benzamidine 4FF

EA02925

1×4.9mL

EA02935

5×4.9mL

EzLoad 16/10 Benzamidine 4FF

EA111304

1 pcs

EzLoad 26/10 Benzamidine 4FF

EA111306

1 pcs

 

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