Hydrophobic interaction chromatography(HIC)is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
Butyl Bestarose 4FF is formed by coupling butyl chain on 4% agarose base matrix. The High physical and chemical stability of agarose base matrix make it have good pressure flow velocity performance, making it an ideal choice for large-scale purification process, especially in the capture or moderate purification stages where a high flow velocity separation process is required. The optimized ligand density is suitable for the separation and purification of biological molecules with strong hydrophobicity.
Appearance |
White slurry, can be layered |
Matrix |
Highly cross-linked agarose, 4% |
Functional group |
Butyl |
Ligand concentration |
~ 40μmol Ligand/mL resin |
Particle size+ |
45~165μm(the average particle size is 90μm) |
Max. pressure |
0.3MPa |
Pressure flow velocity |
≥150cm/h(0.1MPa BXK50/60,H= 25cm,20℃) |
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++、1M HAc++、10mM NaOH、6M GuHCl、8M Urea、30% isopropyl alcohol、70% ethanol、20% ethanol、2%benzyl alcohol |
pH stability |
2~14(CIP),3~13(working) |
Temperature tolerance |
Working temperature: 2~40℃, Can't freeze |
Storage+++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
Recommended flow velocity |
90~150cm/h |
Product |
Code No. |
Pack size |
Butyl Bestarose 4FF |
AH0011 |
25mL |
AH103307 |
100mL |
|
AH103311 |
500mL |
|
AH0013 |
1L |
|
AH0014 |
5L |
|
AH0015 |
10L |
|
AH103315 |
20L |
|
AH103316 |
40L |
Prepacked columns |
Code No. |
Pack size |
EzFast Butyl 4FF |
EH00121 |
1×1mL |
EH002 |
5×1mL |
|
EH103303 |
1×5mL |
|
EH003 |
5×5mL |
|
EzScreen Butyl 4FF |
EH00125 |
1×4.9mL |
EH00135 |
5×4.9mL |
|
EzLoad 16/10 Butyl 4FF |
EH004 |
1 pcs |
EzLoad 26/10 Butyl 4FF |
EH005 |
1 pcs |