CM Bestarose FF

Description

Download

Reviews

1. Introduction

Ion exchange chromatography(IEC)is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

IEC resin is composed of three parts: (1)Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEC resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

CM Bestarose FF is a weak cation exchanger formed by coupling carboxymethyl onto high flow velocity agarose matrix.

2. Technical Characteristics

Appearance	White slurry, can be layered
Matrix	Cross-linked agarose, 6%
Functional group	Carboxy methyl
Particle size⁺	45~165μm
Ionic capacity	90~130μmol H⁺/mL packed resin
Max. pressure	0.3 MPa
Pressure flow velocity++	≥300cm/h（0.1MPa BXK50/30,H=15cm ,20℃,0.1M NaCl）
Chemical Stability	Stable in common aqueous buffers: 1M NaOH+++,1M HAc+++,6M GuHCl,8M Urea, 70% ethanol ,30% isopropyl alcohol, 20% ethanol, 2%benzyl alcohol
pH stability	2~14(CIP),4~13 (Working)
Temperature tolerance	Working temperature:2~40℃, Can't freeze
Storage++++	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommend flow velocity	60~300cm/h
Avoid	Avoid contact with oxidizing agents, cationic detergents. Avoid long time exposures (1 week, 20℃)to pH

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++ The pressure flow velocity describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin. Pressure flow velocity is shown in Fig 1

+++1M NaOH and 1M HAc only be used for cleaning.

++++2% benzyl alcohol is only used for international transport or special requirements from customer

Fig 1. Pressure/flow velocity cure for Bestarose 6FF

3. Order Information

Product	Code No.	Pack size
CM Bestarose FF	AI0041	25mL
	AI0042	100mL
	AI0043	500mL
	AI0044	1L
	AI0045	5L
	AI0046	10L
	AI0047	20L
	AI201316	40L

Prepacked columns	Code No.	Pack size
EzFast CM FF	EI00421	1×1mL
	EI016	5×1mL
	EI201303	1×5mL
	EI017	5×5mL
EzScreen CM FF	EI00425	1×4.9mL
EzScreen CM FF	EI00435	5×4.9mL
EzLoad 16/10 CM FF	EI019	1 pcs
EzLoad 26/10 CM FF	EI020	1 pcs
EzLoad 16/20 CM FF（Double adaptor）	EI051	1 pcs
EzLoad 10/10 CM FF（Double adaptor）	EI054	1 pcs

Share your thoughts Showing

Your rating

Your review *

Name *

Email *

Submit

Weak Cation Exchange Resins

CM Bestarose FF

Product Characteristics

1. Introduction

2. Technical Characteristics

3. Order Information

You Might Also Like...

AT Protein A Diamond Plus

IgM Cap Mustang

Extrem A Diamond

AT Protein A Diamond

Weak Cation Exchange Resins

CM Bestarose FF

Product Characteristics

Related Products

1. Introduction

2. Technical Characteristics

3. Order Information

You Might Also Like...

AT Protein A Diamond Plus

IgM Cap Mustang

Extrem A Diamond

AT Protein A Diamond