DEAE Bestarose FF

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1. Introduction

Ion exchange chromatography(IEC)is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

IEC resin is composed of three parts: (1)Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEC resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

DEAE Bestarose FF(Fast Flow) is a weak anion exchange resin formed by coupling diethylaminoethyl to high-flow agarose microspheres.

2. Technical Characteristics

Appearance	White slurry, can be layered
Matrix	Cross-linked agarose, 6%
Functional Group	Diethylaminoethyl
Particle size+	45~165μm
Ionic capacity	110-160μmol /mL packed resin
Max. pressure	0.3 MPa
Pressure flow velocity++	≥300cm/h（0.1MPa BXK50/30,H=15cm ,20℃,0.1M NaCl）
Chemical stability	Stable in common aqueous buffers: 1M NaOH+++,1M HAC+++, 6M GuHCl,8M Urea, 70% ethanol,30% Isopropyl alcohol, 20% ethanol, 2%Benzyl alcohol
pH stability	2~14(CIP), 2~12 (Working)
Temperature tolerance	Working temperature:2~40℃, Can't freeze
Storage++++	2~30℃，20%ethanol or 2% benzyl alcohol
Recommend flow velocity	60~300cm/h
Avoid	Avoid contact with oxidizing agents, cationic detergents. Avoid long time exposures (1 week, 20℃) to pH

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++ The pressure flow velocity describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin. Pressure flow velocity is shown in Fig 1

+++1M NaOH and 1M HAc only be used for cleaning.

++++2% benzyl alcohol is only used for international transport or special requirements from customer

Fig 1. Pressure/flow velocity cure for Bestarose 6FF

3. Order Information

Product	Code No.	Pack size
DEAE Bestarose FF	AI0031	25mL
	AI0032	100mL
	AI0033	500mL
	AI0034	1L
	AI0035	5L
	AI0036	10L
	AI0037	20L
	AI0038	40L

Prepacked columns	Code No.	Pack size
EzFast DEAE FF	EI00321	1×1mL
	EI011	5×1mL
	EI202303	1×5mL
	EI012	5×5mL
EzScreen DEAE FF	EI00325	1×4.9mL
EzScreen DEAE FF	EI00335	5×4.9mL
EzLoad 16/10 DEAE FF	EI014	1 pcs
EzLoad 26/10 DEAE FF	EI015	1 pcs

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Weak Anion Exchange Resins