DEAE Bestarose HP

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1. Introduction

Ion exchange chromatography(IEC)is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

IEC resin is composed of three parts: (1)Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability; (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEC resin; (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

DEAE Bestarose HP (high performance) is a weak cation exchanger formed by coupling diethylaminoethyl onto high flow velocity agarose matrix.

2. Technical Characteristics

Appearance	White slurry, can be layered
Matrix	Cross-linked agarose, 6%
Functional Group	Diethylaminoethyl
Particle size+	24~44μm (the average particle size is 34μm)
Ionic capacity	110-160 μmol/ mL packed resin
Max. pressure	0.3 MPa
Pressure flow velocity	≥200cm/h (0.1MPa, BXK50/30 ,H=15cm ,25℃)
Working pH range	2~9
Chemical stability	Stable in common aqueous buffers: 1M NaOH++,6M GuHCl,8M Urea, 70% ethanol ,30% isopropyl alcohol
pH Stability	2~14(CIP), 2~12 (Working)
Temperature tolerance	Working temperature:2~40℃, Can't freeze.
Storage+++	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	60~150cm/h
Avoid	Avoid contact with oxidizing agents, cationic detergents. Avoid long time exposures (1 week, 20℃) to pH

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.

++1M NaOH only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
DEAE Bestarose HP	AI0301	25mL
	AI0302	100mL
	AI0303	500mL
	AI0304	1L
	AI0305	5L
	AI0306	10L
	AI0307	20L
	AI0308	40L

Prepacked columns	Code No.	Pack size
EzFast DEAE HP	EI03021	1×1mL
	EI03031	5×1mL
	EI03023	1×5mL
	EI03033	5×5mL
EzScreen DEAE HP	EI03025	1×4.9mL
EzScreen DEAE HP	EI03035	5×4.9mL
EzLoad 16/10 DEAE HP	EI03001	1 pcs
EzLoad 26/10 DEAE HP	EI03011	1 pcs

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Weak Anion Exchange Resins