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Weak Anion Exchange Resins

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Weak Anion Exchange Resins

Diamond DEAE

Product Characteristics

  • Diamond DEAE is a weak anion exchange resin formed by coupling diethylaminoethyl to high-rigidity agarose microspheres.
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1. Introduction 

Ion exchange chromatography(IEC)is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEC resin is composed of three parts: (1)Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability; Traditional agarose molecules are modified to have better mechanical properties, which is called high rigid agarose, or Diamond base matrix. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEC resin; (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Diamond DEAE is a weak anion exchange resin formed by coupling diethylaminoethyl to high-rigidity agarose microspheres.

2. Technical Characteristics

Appearance

White slurry, can be layered

Matrix

Highly-rigidity agarose with long dextran chain

Functional group

Diethylaminoethyl

Average particle size+

90μm

Ionic capacity

290~350 μmol Cl-/mL packed resin

Dynamic binding capacity

> 90mg ovalbumin /mL packed resin

Max. pressure

0.5 MPa

Pressure flow velocity

≥1500cm/h(0.5MPa ,BXK 100/500 ,H=20cm, 20℃)

pH stability

2~9(working)，2~14(CIP)

Chemical stability

Stable in common aqueous buffers: 1M NaOH++,1M HAC++, 6M GuHCl,8M Urea, 30% isopropyl alcohol, 70% ethanol

Avoid contact with oxidizing agents, cationic detergents.

Temperature tolerance

Working temperature:2~40℃, Can't freeze.

Storage+++

2~30℃,20%ethanol or 2% benzyl alcohol

Recommended flow velocity

90~500cm/h
 
+ The average particle size is the cumulative resin particle size of the filler volume distribution.
++1M NaOH and 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product

Code No.

Pack size

Diamond DEAE

AI0121

25mL

AI0122

100mL

AI312311

500mL

AI0123

1L

AI0124

5L

AI0125

10L

AI312315

20L

AI312316

40L
 

Prepacked columns

Code No.

Pack size

EzFast Diamond DEAE

EI01221

1×1mL

EI312351

5×1mL

EI312303

1×5mL

EI312353

5×5mL

EzScreen Diamond DEAE

EI01225

1×4.9mL

EI01235

5×4.9mL

EzLoad 16/10 Diamond DEAE

EI312304

1 pcs

EzLoad 26/10 Diamond DEAE

EI312306

1 pcs
 

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