Diamond Q

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1. Introduction

Ion exchange chromatography(IEC)is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

IEC resin is composed of three parts: (1)Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability; through the modification and modification of traditional agarose molecules, it has stronger mechanical properties, which is called high rigid agarose, namely Diamond substrate; (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEC resin; (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

Diamond Q is a strong anion exchange resin formed by coupling quaternary ammonium groups on highly rigid agarose microspheres, which has stronger pressure resistance and higher load than Q Bestarose FF.

2. Technical Characteristics

Appearance	White slurry, can be layered
Matrix	High-rigidity agarose with a long chain of dextran
Functional group	Quaternary amine group
Average particle size +	90μm
Ionic capacity	160~220μmol Cl^-/mL packed resin
Dynamic binding capacity	> 100mg BSA/mL packed resin
Max. pressure	0.5 MPa
Pressure flow velocity	≥1500cm/h(0.5MPa BXK 100/500 , H =20cm, 20℃)
Chemical Stability	Stable in common aqueous buffers: 1M NaOH⁺⁺,1M HAC⁺⁺,70% ethanol , 20% ethanol, 6M GuHCl,8M Urea Avoid contact with oxidizing agents, cationic detergents.
pH stability	2~14(CIP), 2~12(Working)
Temperature tolerance	Working temperature:2~40℃, Can't freeze, Can tolerate 121℃ high pressure sterilization (20min)
Storage+++	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommend flow velocity	90~500cm/h

+ Average particle size is the accumulated resin particle size of packing volume distribution.

++1M NaOH and 1M HAc only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
Diamond Q	AI0111	25mL
	AI0112	100mL
	AI314311	500mL
	AI0113	1L
	AI0114	5L
	AI0115	10L
	AI314315	20L
	AI314316	40L

Prepacked columns	Code No.	Pack size
EzFast Diamond Q	EI01121	1×1mL
	EI314351	5×1mL
	EI314303	1×5mL
	EI314353	5×5mL
EzScreen Diamond Q	EI01125	1×4.9mL
EzScreen Diamond Q	EI01135	5×4.9mL
EzLoad 16/10 Diamond Q	EI314304	1 pcs
EzLoad 26/10 Diamond Q	EI314306	1 pcs

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