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Strong Cation Exchange Resins

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Ion Exchange Resins
Strong Cation Exchange Resins

Diamond SP

Product Characteristics

  • Diamond SP is a strong cation exchange resin formed by coupling sulfopropyl on high rigid agarose microspheres, which has better pressure resistance and higher load than SP Bestarose FF.

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1. Introduction 

Ion exchange chromatography(IEC)is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEC resin is composed of three parts: (1)Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability; (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEC resin; (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Diamond SP is a strong cation exchange resin formed by coupling sulfopropyl on high rigid agarose microspheres, which has better pressure resistance and higher load than SP Bestarose FF.

2. Technical Characteristics

Appearance

White slurry, can be layered

Matrix

Highly rigid agarose containing long chains of dextran

Functional group

Sulfopropyl

Average particle size+

90μm

Ionic capacity

170~220 μmol H+/mL packed resin

Dynamic binding capacity

> 100 mg lysozyme/mL packed resin

Max. pressure

0.5MPa

Pressure flow velocity

≥1500cm/h(0.5MPa, BXK 100/500 ,H=20cm, 20℃)

Chemical stability

Stable in common aqueous buffers: 1M NaOH++, 1M HAc++ 、6M GuHCl, 30% isopropyl alcohol, 70% ethanol

Avoid contact with oxidizing agents, cationic detergents.

pH stability

3~14(CIP)，4~13(working)

Temperature tolerance

Working temperature:2~40℃, Can't freeze.

Storage+++

2~30℃, 20% ethanol with 0.2M NaAc or 2% benzyl alcohol with 0.2M NaAc

Recommended flow velocity

90~500cm/h
 
+ Average particle size is the accumulated resin particle size of packing volume distribution
++1M NaOH and 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product

Code No.

Pack size

Diamond SP

AI319305

25mL

AI319307

100mL

AI319311

500mL

AI319312

1L

AI319313

5L

AI319314

10L

AI319315

20L

AI319316

40L
 

Prepacked columns

Code No.

Pack size

EzFast Diamond SP

EI04521

1×1mL

EI04531

5×1mL

EI04523

1×5mL

EI04533

5×5mL

EzScreen Diamond SP  

EI04525

1×4.9mL

EI04535

5×4.9mL

EzLoad 16/10 Diamond SP

EI04501

1 pcs

EzLoad 26/10 Diamond SP

EI04511

1 pcs

 

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