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MegaPoly AT Analytical antibody column

Product Characteristics

  • Monodisperse polystyrene-divinylbenzene microspheres made base matrix, better resistance towards high pressure, higher flow rate.
  • Hydrophilizing treatment on surface, minimized non-specific adsorption, ensuring accuracy in testing results.
  • Designed for high pressure column, suitable for HPLC operation.
  • Alkali-resistant protein A ligand enables selective binding with IgG and Fc fusion protein, ensuring accuracy in testing results.
  • Operation at 2mL/min normal flow rate and 4mL/min maximum flow rate, significant improvements in analysis efficiency.
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1. Introduction 

MegaPoly AT is antibody analytic column used for the HPLC system at high flow rate and high pressure. Its pre-packed resin uses porous polystyrene-divinylbenzene microspheres as its base matrix, which is coupled on alkali-resistant Protein A ligand after hydrophilic treatment on its surface. The resin can efficiently bind with IgG and Fc fusion protein, being applicable for the quantitative analysis and small-scale purification of antibodies and fusion proteins.
MegaPoly AT features:
Monodisperse polystyrene-divinylbenzene microspheres made base matrix, better resistance towards high pressure, higher flow rate.
Hydrophilizing treatment on surface, minimized non-specific adsorption, ensuring accuracy in testing results.
Designed for high pressure column, suitable for HPLC operation
Alkali-resistant protein A ligand enables selective binding with IgG and Fc fusion protein, ensuring accuracy in testing results.
Operation at 2mL/min normal flow rate and 4mL/min maximum flow rate, significant improvements in analysis efficiency.


2. Technical Characteristics

Physical appearance

White slurry, can be layered after placing

Base matrix

polystyrene-divinylbenzene microspheres

Average particle size

20μm

Functional groups

Alkali resistant Protein A

IgG binding capacity

~35mg/mL

Chemical stability

pH2-9：6M CH6ClN3, 8M urea,20% ethanol, 0.5M NaOH

pH stability

2~10( work )

Temperature tolerance

2~40℃，no freezing

Max pressure

20MPa

Storage

2~8℃，20% ethanol

Max flow rate

4mL/min
 

 

Particle size

μm

Column volume

ml

Inner diameter×bed height

mm×mm

Column body material

Recommended flow rate+

Storage solution++

20

0.1

2.1×30

PEEK

2ml/min

20% ethanol
 

 

+ Recommended flow rate is used for operation, Minimum flow rate or 2ml/min flow rate is used for CIP and organic solution.
++ degas is necessary for 20% ethanol 

3. Order Information

Column

Column volume

Material

Item code

Pack size

MegaPoly AT

0.1mL

PEEK

AC002

1 pcs
 

 

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