Diamond Layer 700 BA

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1. Introduction

Diamond Layer 700 BA is novel mixed-mode purification chromatography resin designed for intermediate purification and polishing purification of viruses and other biomolecules.

The resin has a two-layer structure, nucleic microspheres and a thin shell. The nucleic microspheres are prepared by crosslinking the benzylamine ligands to high rigidity agarose, and have anionic and hydrophobic effects. The thin shell is a layer of agarose structure without ligand, which has a molecular sieve function. In the purification process, large molecules such as viruses cannot enter the inside of the microsphere through the shell, so they flow through directly. Most impurities can enter the inside of the microsphere and bind to the resin through ion and hydrophobic interaction. These characteristics make Diamond Layer 700 BA resin an excellent choice for virus purification resin in vaccine production. The size exclusion of outer layer is 700KD. Biomolecules smaller than the corresponding molecular weight could enter the microsphere and bond with ligand groups, while biomolecules bigger than the corresponding molecular weight would be blocked in the microsphere outflow and pass through the chromatography column.

The main characteristics of Diamond Layer 700 BA resin include:

The double-layer structure of the microspheres enables the resin to effectively adsorb impurities while the target protein flows through to achieve the purification effect.

Due to the flow through of the target, the impurities are bond in hydrophobic and ionic mode, allowing relatively wide operating conditions.

Compared with the conventional molecular sieve resin, the high sample loading volume and high flow velocity improve the production efficiency.

2. Technical Characteristics

Matrix	Highly cross-linked rigidity agarose
Functional group	Benzylamine
Average particle size⁺	85μm
Appearance++	White to slight yellow slurry
Dynamic binding capacity	~13mg ovalbumin/mL packed resin
Average molecular weight exclusion	Mr≈700 000
Pressure flow velocity	≥500cm/h(H=20cm，pressure＜2bar)
Chemical Stability	Stable in common aqueous buffers: 1M NaOH+++,1M HAC+++,70% ethanol , 20% ethanol , 30% isopropyl alcohol, 6M GuHCl, 0.5% twain 80, 1M NaOH+30% isopropyl alcohol
pH stability	3~13(working), 2~14(CIP)
Storage++++	2~30℃, 20% ethanol or 2% benzyl alcohol
Avoid	Avoid contact with oxidizing agents, citrate buffer solution.

+ Average particle size is the accumulated resin particle size of packing volume distribution.

++Due to ligand properties, the color gradually changes from white to light yellow with the increase of storage time, and the color change

has no effect on product performance.

+++1M NaOH and 1M HAc only be used for cleaning.

++++2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order Information

Product	Cat. No.	Pack size
Diamond Layer 700 BA	AI04901	25mL
	AI04902	100mL
	AI04903	500mL
	AI04904	1L
	AI04905	5L
	AI04906	10L
	AI04907	20L

Prepacked columns	Cat. No.	Pack size
EzFast Diamond Layer 700 BA	EI04921	1×1mL
	EI04931	5×1mL
	EI04923	1×5mL
	EI04933	5×5mL
EzScreen Diamond Layer 700 BA	EI04925	1×4.9mL
EzScreen Diamond Layer 700 BA	EI04935	5×4.9mL
EzLoad 16/10 Diamond Layer 700 BA	EI04901	1 pcs
EzLoad 26/10 Diamond Layer 700 BA	EI04911	1 pcs

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Diamond Layer 700 BA