Ni Bestarose FF

Product Characteristics

Large adsorption capacity, good selection type, easy regeneration, and low cost.

Ni Bestarose HP

IMAC Bestarose FF

Description

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1. Introduction

Ni Bestarose FF(Fast Flow)metal chelation chromatography resin is an affinity chromatography resin that pre-chelates metal ion Ni2+ on agarose gel with NTA as ligand. It has the advantages of large adsorption capacity, good selection type, easy regeneration, and low cost. It is widely used in the separation and purification of downstream proteins and peptides in biopharmaceutical and bioengineering, especially the efficient purification of histidine-tagged proteins.

2. Technical Characteristics

Appearance	Turquoise slurry, can be layered
Matrix	Highly Cross-linked agarose, 6%
Particle size⁺	45~165μm
Ligand concentration	12~18μmol Ligand/mL resin
Dynamic binding capacity	~ 40mg His tag protein/ mL packed resin
Chemical stability ⁺⁺	40℃ One week : 10mM HCl, 0.1M NaOH, 6M GuHCl,8M Urea; 40℃ 12h: 1M NaOH, 70% acetic acid;
pH stability⁺⁺⁺	3~12 (working) 2~14 (CIP)
Pressure flow velocity	~600cm/h，BXK16/20，H=5cm，25℃
Max. pressure	0.3MPa
Storage⁺⁺⁺⁺	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommend flow velocity	＜150cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++ Stability when removing metal ions

+++ CIP refers to pH stability when metal ions are removed.

++++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
Ni Bestarose FF	AA0051	25mL
	AA0052	100mL
	AA0053	500mL
	AA0054	1L
	AA0055	5L
	AA0056	10L
	AA208315	20L

Prepacked columns	Code No.	Pack size
EzFast Ni FF	EA208301	1×1mL
	EA208303	1×5mL
	EA006	5×1mL
	EA007	5×5mL
EzScreen Ni FF	EA00525	1×4.9mL
EzScreen Ni FF	EA00535	5×4.9mL
EzLoad 16/10 Ni FF	EA208304	1 pcs
EzLoad 26/10 Ni FF	EA208306	1 pcs

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Tag Affinity Resins

Ni Bestarose FF

Product Characteristics

1. Introduction

2. Technical Characteristics

3. Order Information

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Ni Bestarose FF

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1. Introduction

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3. Order Information

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