Phenyl Bestarose HP

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1. Introduction

Hydrophobic interaction chromatography(HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Phenyl Bestarose HP is based on highly cross-linked agarose, which retains the excellent hydrophilicity and pore structure of natural polysaccharide compounds and has good compatibility with biological macromolecules. The average particle size of Phenyl Bestarose HP is 34μm, which has a higher resolution efficiency than Phenyl Bestarose FF. The surface contains hydrophobic ligands, the ligand is butyl, which is particularly suitable for the fine separation and purification of recombinant proteins, antibodies, vaccines, VLP particles and other highly hydrophobic biological molecules.

2. Technical Characteristics

Appearance	White slurry, can be layered
Matrix	Cross-linked agarose, 6%
Particle size+	24~44μm(the average particle size is 34μm)
Functional group	Phenyl
Ligand concentration	~ 25μmol Ligand/mL resin
Dynamic binding capacity	~ 45mg α-Chymotrypsinogen /mL packed resin
Max. pressure	0.3 MPa
Pressure flow velocity	≥200cm/h(0.1MPa,BXK 50/30 H=15cm, 25℃)
Chemical stability	Stable in common aqueous buffers: 1M NaOH++, 1M HAc++, 6M GuHCl, 30% isopropyl alcohol, 70% ethanol
pH stability	3~13(CIP), 3~12(working)
Temperature tolerance	Working temperature: 2~40℃, Can't freeze, Can tolerate 121℃ high pressure sterilization (20min)
Storage+++	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	60-120cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.

++1M NaOH and 1M HAc only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
Phenyl Bestarose HP	AH201105	25mL
	AH201107	100mL
	AH201111	500mL
	AH0042	1L
	AH0043	5L
	AH0044	10L
	AH201115	20L
	AH201116	40L

Prepacked columns	Code No.	Pack size
EzFast Phenyl HP	EH00421	1×1mL
	EH017	5×1mL
	EH201103	1×5mL
	EH018	5×5mL
EzScreen Phenyl HP	EH00425	1×4.9mL
EzScreen Phenyl HP	EH00435	5×4.9mL
EzLoad 16/10 Phenyl HP	EH019	1 pcs
EzLoad 26/10 Phenyl HP	EH020	1 pcs

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Phenyl HIC Resins