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Strong Anion Exchange Resins

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Strong Anion Exchange Resins

Q Bestarose BB

Product Characteristics

  • Q Bestarose BB is a strong anion exchange resin, which can capture biological macromolecules directly from the feed liquid, and its fast flow velocity is suitable for large-scale production applications.
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1. Introduction 

Ion exchange chromatography(IEC)is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEC resin is composed of three parts: (1)Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability; (2)Functional groups fixed to the matrix, which are electrically charged groups, determining the properties of the IEC resin; (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
The Big Beads series resin are coupled with various functional groups on the agarose base frame with high particle crosslinking. The large particle base can tolerate samples with poor clarity, it is not easy to plug the column, and the flow velocity is fast, suitable for processing large samples in a short time.
Q Bestarose BB is a strong anion exchange resin, which can capture biological macromolecules directly from the feed liquid, and its fast flow velocity is suitable for large-scale production applications.

2. Technical Characteristics

Appearance

White slurry, can be layered

Matrix

Highly crosslinked agarose,6%

Functional group

Quaternary amine group

Particle size +

100~300μm

Ionic capacity

180~250 μmol Cl-/mL packed resin

The exclusion limit, Mr

~4 × 106 (globular protein)

Max. pressure

0.3 MPa

Chemical stability

Stable in common aqueous buffers: 1M NaOH++,6M GuHCl,30% isopropanol,70% ethanol

Avoid contact with oxidizing agents.

pH stability

2~14(CIP),2~12 (working)

Temperature tolerance

Working temperature:2~40℃, Can't freeze.

Storage+++

2~30℃,20%ethanol or 2% benzyl alcohol

Recommend flow velocity

300~900cm/h

 
+Particle size is normally distributed, and particles within this range account for more than 80% of the total.
.    ++1M NaOH only be used for cleaning.
    +++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product

Code No.

Pack size

Q Bestarose BB

AI204405

25mL

AI204407

100mL

AI204411

500mL

AI204412

1L

AI204413

5L

AI204414

10L

AI204415

20L

AI204416

40L

 

Prepacked columns

Code No.

Pack size

EzFast Q BB

EI02921

1×1mL

EI204451

5×1mL

EI204403

1×5mL

EI204453

5×5mL

EzScreen Q BB

EI02925

1×4.9mL

EI02935

5×4.9mL

EzLoad 16/10 Q BB

EI204404

1 pcs

EzLoad 26/10 Q BB

EI204406

1 pcs

 

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