Bestarose 4FF&Bestarose 6FF

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1. Introduction

Bestarose 4FF(Fast Flow)and Bestarose 6FF are gel filtration resin made by emulsification, rinsing and sieving of 4% and 6%，respectively. They are used for separation, purification, or detection of biological macromolecules such as polysaccharides, nucleic acids and viruses. This resin is often used as a matrix for affinity, hydrophobic and ion exchange chromatography. Bestarose 4FF and Bestarose 6FF are crosslinked with bifocal crosslinkers based on the gels of Bestarose 4B and Bestarose 6B, and the degree of crosslinking is higher than that of Bestarose CL-4B and Bestarose CL-6B.More stable physical and chemical properties, this kind of resin can withstand 121°C autoclave, fast flow velocity, suitable for large-scale separation and purification applications.

2. Technical Characteristics

Product		Bestarose 4FF	Bestarose 6FF
Appearance		White slurry, can be layered
Agarose concentration		4%	6%
Separation range	Linear numerator	30KD~5000KD	10KD~1000KD
	Globular molecule	60KD~20000KD	10KD~4000KD
Particle size +		45-165μm
Average particle size		90μm
Pressure flow velocity⁺⁺		200-300cm/h（0.1MPa BXK50/60，H=25cm，20°C，in water）	≥200cm/h（0.1MPa BXK50/60，H=25cm，20°C，in water）
Max. pressure		0.3MPa(3bar)
pH stability		2~12(working),2~14(CIP)
Chemical stability		Stable in common aqueous buffers: 0.5M NaOH+++,2M NaOH+++,6M GuHCl,8M Urea, 1M acetic acid, 30% acetonitrile	Stable in common aqueous buffers: 0.5M NaOH+++,2M NaOH+++,1M acetic acid, 30% acetonitrile, 8M Urea, 6M GuHCl
Sterilization		Can tolerate 121℃ high pressure sterilization (20min)
Storage++++		2~30℃, 20% ethanol or 2% benzyl alcohol

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++ The pressure flow velocity describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin

+++0.5M NaOH and 2M NaOH only be used for cleaning.

++++ 2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
Bestarose 4FF	AG0052	100mL
	AG0053	500mL
	AG0054	1L
	AG0055	5L
	AG0056	10L
	AG2157	20L
	AG214316	40L
Bestarose 6FF	AG0062	100mL
	AG0063	500mL
	AG0064	1L
	AG0065	5L
	AG0066	10L
	AG2167	20L
	AG215316	40L

Prepacked columns	Code No.	Pack size
EzLoad 16/60 Bestarose 4FF	EG214301	1 pcs
EzLoad 26/60 Bestarose 4FF	EG214302	1 pcs
EzLoad 16/90 Bestarose 4FF	EG027	1 pcs
EzLoad 26/90 Bestarose 4FF	EG00513	1 pcs
EzLoad 16/60 Bestarose 6FF	EG215301	1 pcs
EzLoad 26/60 Bestarose 6FF	EG215302	1 pcs
EzLoad 16/90 Bestarose 6FF	EG028	1 pcs
EzLoad 26/90 Bestarose 6FF	EG00613	1 pcs

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Agarose Based Resins