Diamond Layer 400 & Layer 700

Description

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Application

1. Introduction

Diamond Layer 400 & Diamond Layer 700 are novel multimodal purification chromatography resin designed for intermediate purification and fine purification of viruses and other biomolecule.

The resin has a two-layer structure, nucleic microspheres and a thin shell. The nucleic microspheres are prepared by crosslinking the octylamine ligands to high rigid agarose, and have anionic and hydrophobic effects. The thin shell is a layer of agarose structure without ligand, which has a molecular sieve function. In the purification process, large molecules such as viruses cannot enter the inside of the microsphere through the shell, so they flow through directly. Most impurities can enter the inside of the microsphere and bind to the resin through ion and hydrophobic interaction. These characteristics make Diamond Layer 400 and Diamond Layer 700 resin an excellent choice for virus purification resin in vaccine production. The size cut-off of outer layer was 400KD and 700KD, respectively. Biomolecules less than the corresponding molecular weight could enter the microsphere and bond with ligand groups, while biomolecules greater than the corresponding molecular weight would be blocked in the microsphere outflow and pass through the chromatography column.

The main characteristics of Diamond Layer 400 and Diamond Layer 700 resin include:

The double-layer structure of the microspheres enables the resin to effectively adsorb impurities while the target protein flows through to achieve the purification effect.

Due to the flow through of the target, the impurities are combined in hydrophobic and ionic mode, and the operating conditions are relatively wide.

Compared with the conventional molecular sieve resin, the high sample loading volume and high flow velocity improve the production efficiency.

2. Technical Characteristics

Product	Diamond Layer 400	Diamond Layer 700
Matrix	Highly cross-linked rigid agarose
Functional group	Octylamine
Average particle size +	90μm	85μm
Dynamic binding capacity	~22mg ovalbumin/mL packed resin	~13mg ovalbumin/mL packed resin
Average molecular weight exclusion	Mr≈400 000	Mr≈700 000
Pressure flow velocity	≥700cm/h，	≥500cm/h，
Chemical Stability	Stable in common aqueous buffers: 1M NaOH++,1M HAC++,70% ethanol , 20% ethanol , 30% isopropyl alcohol, 6M GuHCl, 0.5% twain 80, 1M NaOH+30% isopropyl alcohol
pH stability	3~13(working)，2~14(CIP)
Storage+++	2~30℃, 20% ethanol or 2% benzyl alcohol
Avoid	Avoid contact with oxidizing agents, cationic detergents.

+ Average particle size is the accumulated resin particle size of packing volume distribution

++1M NaOH and 1M HAc only be used for cleaning.

+++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order Information

Product	Code No.	Pack size
Diamond Layer 400	AI0471	25mL
	AI0472	100mL
	AI0473	500mL
	AI0474	1L
	AI0475	5L
	AI0476	10L
	AI0477	20L
	AI0478	40L
Diamond Layer 700	AI0461	25mL
	AI0462	100mL
	AI0463	500mL
	AI0464	1L
	AI0465	5L
	AI0466	10L
	AI0467	20L
	AI0468	40L

Prepacked columns	Code No.	Pack size
EzFast Diamond Layer 400	EI04721	1×1mL
	EI04723	5×1mL
	EI04731	1×5mL
	EI04733	5×5mL
EzScreen Diamond Layer 400	EI04725	1×4.9mL
EzScreen Diamond Layer 400	EI04735	5×4.9mL
EzLoad 16/10 Diamond Layer 400	EI04701	1 pcs
EzLoad 26/10 Diamond Layer 400	EI04711	1 pcs
EzFast Diamond Layer 700	EI04621	1×1mL
	EI04623	5×1mL
	EI04631	1×5mL
	EI04633	5×5mL
EzScreen Diamond Layer 700	EI04625	1×4.9mL
EzScreen Diamond Layer 700	EI04635	5×4.9mL
EzLoad 16/10 Diamond Layer 700	EI04601	1 pcs
EzLoad 26/10 Diamond Layer 700	EI04611	1 pcs

Diamond Layer 700 BA