CNBr-activated Bestarose 4B

Product Characteristics

Mild reaction conditions with protein and other biological macromoleculesCan be directly coupled to biological macromolecules without coupling spacersThe ligand and the scaffold can form a multi-site coupling, the ligand should not fall off

NHS-activated Bestarose 4FF

Description

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1. Introduction

CNBr-activated Bestarose 4B is a pre-activated agarose gel with cyanogen bromide.Biomolecules with a primary amino group (-NH2) can be covalently coupled to this resin for making specific affinity resin and immobilized enzymes.

The resin has the following characteristics:

Mild reaction conditions with protein and other biological macromolecules

Can be directly coupled to biological macromolecules without coupling spacers

The ligand and the scaffold can form a multi-site coupling, the ligand should not fall off

Applied to the coupling of proteins, peptides, nucleic acids, etc.

2. Technical Characteristics

Appearance	White slurry，can be layered /white powder solid
Matrix	Cross-linked 4% agarose
Particle size⁺	45~165μm
Conjugated functional groups	Primary amino group
Amount of conjugated protein	25-60mg α-chymotrypsinogen/mL resin
pH stability++	3~11(working) 2~11(CIP)
Chemical stability+++	Stable in common aqueous buffers: 6M GuHCl,8M Urea, 70% ethanol
Max. flow velocity	≥15cm/h(BXK26/40，H=30cm, 20℃，in water)
Max.pressure	0.16bar
Storage	2 ~ 8℃, 100% acetone

+ The particle size is normally distributed, and the particles in this range account for more than 95% of the total

++ No coupling ligand

+++ Data when the coupled ligand is also stable

3. Order Information

Product	Code No.	Pack size
CNBr-activated Bestarose 4B	AA017305	25mL
	AA017307	100mL
	AA0012	500mL

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Pre-activated Resins