NHS-activated Bestarose 4FF

Product Characteristics

Easy to form amide bond with protein, strong chemical stabilityThe extension arm with 10 carbon atoms reduces the steric hindrance of ligand and agarose surfaceEasy operation, avoid using highly toxic raw materials，Fast flow velocity, suitable for large-scale applications

CNBr-activated Bestarose 4B

Description

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1. Introduction

NHS-activated Bestarose 4FF is a pre-activated agarose gel, the matrix is high-flow agarose Bestarose 4FF, with spacer arms suitable for coupling small molecule ligands.

The pre-activation resin has the following characteristics:

Easy to form amide bond with protein, strong chemical stability

The extension arm with 10 carbon atoms reduces the steric hindrance of ligand and agarose surface

Easy operation, avoid using highly toxic raw materials

Fast flow velocity, suitable for large-scale applications

2. Technical Characteristics

Appearance	White slurry, can be layered
Matrix	Highly cross-linked agarose, 4%
Particle size+	45~165μm
Activating group	N-hydroxysuccinimide
Ligand concentration	16~23μmol NHS /mL resin
Coupling functional group	-NH2
Max. pressure	0.3MPa
Pressure flow velocity	~150cm/h（0.1MPa BXK50/60 H=25cm 25°C）
Chemical stability++	Stable in common aqueous buffers: 6M GuHCl,8M Urea, 70% ethanol
pH stability+++	3~13(working), 2~13(CIP)
Storage	2~8℃, 100% isopropyl alcohol
Recommended flow velocity	150~300cm/h

+ The particle size is normally distributed, and the particles in this range account for more than 95% of the total

++Data when the coupled ligand is also stable

+++ No coupling ligand

3. Order Information

Product	Code No.	Pack size
NHS-activated Bestarose 4FF	AA0031	25mL
	AA118307	100mL
	AA0032	500mL

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Pre-activated Resins