Understand Ultra performance by one article

Jul 25,2024

Bestchrom

Background information

Last week, we introduced application case of AT Protein A Diamond Ultra resin launched by Bestchrom.

Case study one: Efficient purification of bi-specific antibody 

· Sample information: bi-specific antibody, molecular weight 148KDa

· Column: EzScreen 4.3mL, column bed height : 10cm

· Flow: 0.7 mL/min

· Buffer: PB and similar buffer, pH7.2

· Elution buffer: NaAc-HAc and similar buffer, pH 3.3

Dynamic binding capacity data

In this experiment, DBC of bi-specific antibody detection has been carried out on 12 types of resins available on the market. Get the DBC @ 10% break-through(see the following picture). As illustrated, DBC of AT Protein A Diamond Ultra can reach 43.88mg/mL, which is higher than that of resins from other brands. This enables larger sample volume being processed, boosting productivity.

Fig.1 DBC data of resins

Purification process and results

After comparing sample purity before/after purification, it is found that AT Protein A Diamond Ultra can effectively remove impurities and improving purity of target antibody. See the following figure for chromatogram and testing data.

Fig. 2 Chromatogram of antibody purification

Fig. 4 Purification results data

Case study two: Efficient purification of monoclonal antibody

· Sample information: monoclonal antibody molecule, molecular weight 148KDa

· Column: EzScreen 4.3 mL, column bed height 10cm

· Flow: 0.7 mL/min

· Equilibration buffer: Tris-HCl and similar buffers, pH 7.2

· Cleaning buffer: citric acid and similar buffers, pH 5.5

Dynamic binding capacity data

In this experiment, monoclonal antibody was loaded on three types of affinity resins from Bestchrom. Get DBC @ 10% break-through data(see the following figure). As illustrated, Extrem A Diamond enjoyed the highest DBC(71.63mg/mL), while AT Protein A Diamond Ultra resin reached a DBC of 69.44mg/mL, a level close to Extrem A Diamond.

Fig.3 DBC of mAb @ 10% BT

Purification process and results

After comparing sample purity before/after purification, it is found that AT Protein A Diamond Ultra can effectively remove impurities and improving purity of target antibody. See the following figure for chromatogram and testing data.

Fig.4 Chromatogram of monoclonal antibody purification

Fig. 5 Purification results data

As illustrated by the application cases and data, AT Protein A Diamond Ultra enjoys exceptional performance in purification applications. From bench-scale lab experiment to process-scale purification project, the resin shows consistent performance stability and excellent purification productivity.

As application cases increase and feedbacks accumulate, AT Protein A Diamond Ultra will play a more vital role in bio-processing sector. The future will undoubtedly witness more technical break-through and innovation brought by Bestchrom to bio-pharmaceutical industry.