Bestchrom antibody analytical column formed by coupling alkaline resistant ligands on polystyrene-divinylbenzene resin, which is applicable for the rapid quantitative preparation and micro-preparation of monoclonal antibodies and Fc fusion proteins. It enjoys great convenience and efficiency in operation, enabling sample testing completion in minutes. In addition, it enjoys excellent linear relation within certain concentration range. All these factors enable its wide application in cell culture condition selection during R&D as well as quality control in manufacturing process.