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Chromatography media (also known as "chromatography resin" when used in the field of biological macromolecules) is the core key of liquid chromatography technology. It has a wide range of applications and plays a vital role in the results and efficiency of separation and purification, especially In the field of biopharmaceuticals, chromatography media/chromatography fillers and their processes are the most important factors affecting chromatography/chromatography efficiency.
The separation and purification process not only directly affects the quality of the finished product, but also accounts for the main cost of the entire biopharmaceutical production process.
Chromatography media, also known as chromatography resins or adsorbents, are widely used in various industries. Some major uses of chromatography media include:
Protein Purification: Chromatography resins are widely used in the biopharmaceutical industry to purify proteins, antibodies, enzymes and other biomolecules.
Drug Development: Chromatographic resins play a vital role in the drug discovery and development process. They are used to purify and analyze potential drug candidates, separate impurities, and characterize drug-protein interactions.
Environmental Analysis: Chromatographic media are used to analyze environmental samples such as air, water, soil, and sediments. Contaminants, pesticides, organic compounds and heavy metals in environmental samples can be detected and quantified.
Food and Beverage Industry: Chromatographic media are used in the food and beverage industry for quality control and analysis. Resins are used to separate and quantify nutrients, additives, flavors, contaminants and other components present in food.
Pharmaceutical Analysis: Chromatography media plays a vital role in pharmaceutical analysis to quantify active pharmaceutical ingredients (APIs), excipients, degradation products, and impurities in pharmaceutical formulations.
The core-shell chromatographic medium has its unique advantage that the same medium can be divided into two parts: the core and the shell. The two parts can be made of materials with different characteristics, making the medium have different characteristics. Common putamen-type media are based on natural polysaccharides.
Natural polysaccharides have a large number of hydroxyl groups, which facilitates the separation and purification of target products in an aqueous environment. At the same time, the hydroxyl groups can also undergo chemical reactions to prepare new chromatographic media.
This type of medium mainly enhances the adsorption effect of the medium by increasing the length of the spacer arm (short spacer arm and long spacer arm) and the effective groups on the surface of the medium.
It expands the space inside the medium and reduces the steric hindrance to the diffusion of separated substances in the medium. This increases the diffusion rate of the separated substances within the medium to a certain extent and facilitates the diffusion of the target separated substances into the medium to achieve adsorption of target substances.
The main feature of this type of media is that there are two different types of pores inside the same medium, one is a larger circulation pore, and the other is a small diffusion pore. Due to the existence of the dual-pore structure, the separation of substances is greatly accelerated.
The mass transfer inside the medium enables the separated substances to diffuse better inside the medium, which increases the exchange opportunities between the target substance and the groups on the surface of the medium, allowing the target protein to exchange more groups with the groups inside the medium. , achieves the effect of adsorption and separation, and improves the adsorption capacity of the medium to a certain extent.
This is the most commonly used chromatography medium for classifying proteins. It separates proteins in a sample based on their charge. If a negatively charged resin is used to capture positively charged molecules, the method is called cation exchange chromatography. On the contrary, the resin is positively charged and the target molecules are negatively charged, which is called anion exchange chromatography.
In size-exclusion chromatography (SEC), a sample is filtered through a gel made of spherical beads with pores of a specific size. SEC gels consist of spherical beads containing pores of specific sizes to include or exclude molecules within the medium. Separation occurs as the sample passes through the column and elutes in order of decreasing molecular weight.
Affinity separation is a very precise separation that almost always captures only one protein in the mixture. Affinity chromatography (AC) exploits the strong but reversible interaction between sample proteins and specific ligands to isolate target molecules. This binding interaction immobilizes the ligand to the resin along with the target compound.
| GE Healthcare Life Sciences | GE Healthcare Life Sciences is a major manufacturer and supplier of chromatography resins for biopharmaceutical applications. |
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| Bio-Rad Laboratories | Bio-Rad Laboratories offers a range of chromatography resins for the purification and separation of proteins, antibodies, and other biomolecules. |
| Merck KGaA | Merck KGaA provides a variety of chromatography resins for the purification and separation of proteins, antibodies, and other biomolecules. |
| Mitsubishi Chemical Corporation | Mitsubishi Chemical Company (MCC) is a multinational chemical corporation that manufactures a diverse range of products, including chromatography resins. |
| Purolite Corporation | Purolite Corporation is a leading global producer and supplier of high-quality chromatography resins for use in biopharmaceutical, food and beverage, and other industrial applications. |
| Tosoh Corporation | Tosoh Corporation is a Japanese chemical firm that manufactures a variety of products, including chromatography resins. |
| Thermo Fisher Scientific | Thermo Fisher Scientific's chromatography resins are designed to deliver high purity, yield, and throughput and are suited for usage in a variety of applications such as drug discovery, bioprocessing, and clinical diagnostics |
| Pall Corporation | Pall Corporation specializes in the development of chromatographic resins for biopharmaceutical manufacturing. |
| Repligen Corporation | One of its primary areas of competence is the development of chromatography resins, which are utilized in biologic drug purification. Protein A, Protein G, and Protein L resins from Repligen Corporation are commonly used in the purification of monoclonal antibodies. |
| Bestchrom (China) | Bestchrom, Inc. is a Chinese supplier of high-quality chromatography resins to customers around the world. Bestchrom offers a wide range of chromatography resins for the biopharmaceutical industry. |
The global chromatography resin market size was valued at USD 2.8 billion in 2023 and is projected to reach USD 4.1 billion by 2028, growing at 8.2% cagr during the forecast period.
The market growth of chromatography resins is propelled by two key factors: the rising demand for biosimilars and the continuous expansion of pharmaceutical and biopharmaceutical R&D activities. Particularly, the growing demand for biosimilars in the Asia Pacific region is driving the need for chromatography resins, while further growth is anticipated in the US and Japan.
Source: Chromatography Resin Market Size & Share Report
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