AT Protein A Diamond Ultra

Description

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1. Introduction

AT Protein A Diamond Ultra is an innovative affinity resin made by coupling alkali-resistant Protein A with a high-rigidity agarose matrix. The optimized resin pore size helps with the increasing in binding capacity of the antibody. The resin is suitable for capturing monoclonal antibodies or Fc fusion proteins from a large amount of cell culture media, as well as polyclonal antibodies from ascites fluid or plasma.

The resin can be cleaned in place with 0.1-0.5M NaOH, which avoids the use of expensive and corrosive cleaning reagents, effectively cutting costs. In addition, compared with AT Protein A Diamond Plus, the new resin enjoys higher binding capacity, as well as lower production cost due to the smaller CV selected in scale-up process.

The resin has the following characteristics:

High rigidity, low back pressure and fast flow velocity, suitable for large-scale production applications.

High binding capacity, reduce production costs.

Good alkaline tolerance, longer service life.

2. Technical characteristics

Appearance	White slurry
Matrix	High rigidity agarose
Particle size⁺	40~100μm
Functional group	Recombinant Alkali Tolerant Protein A
Cross-linking method	Epoxy chemistry
Dynamic binding capacity⁺⁺	≥75mg Human IgG/mL packed resin(Residence time 6min)
Chemical stability	Stable in common aqueous buffers: 10mM HAc, 0.1M sodium citrate, 6M GuHCl, 8M Urea, 30% isopropyl alcohol, 20% ethanol.
Max. pressure	0.5MPa
Pressure flow velocity	≥300cm/h,<2bar,BXK300/500,H=20cm
pH stability	3~12(working) 2~13(CIP) CIP: 0.1~0.5M NaOH
Storage⁺⁺⁺	2~8℃, 20% ethanol or 2% benzyl alcohol

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.

++Linear flow velocity 100cm / h, column height 20cm, buffer conditions: 20mM PB, 0.15M NaCl, pH7.4.

+++2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
AT Protein A Diamond Ultra	AA05701	25mL
	AA05702	100mL
	AA05703	500mL
	AA05704	1L
	AA05705	5L
	AA05706	10L

Prepacked columns	Cat. No.	Pack size
EzFast AT Protein A Ultra	EA05721	1×1mL
	EA05731	5×1mL
	EA05723	1×5mL
	EA05733	5×5mL
EzScreen AT Protein A Ultra	EA05725	1×4.6mL
EzScreen AT Protein A Ultra	EA05735	5×4.6mL
EzSelect AT Protein A Ultra	EA05726	8×600μL
EzLoad 16/10 AT Protein A Ultra	EA05701	1pcs
EzLoad 26/10 AT Protein A Ultra	EA05711	1pcs