Protein A Affinity Resins

1. Introduction

AT Protein A Diamond Plus is an innovative affinity resin made by coupling alkali-resistant Protein A with a high-rigidity agarose matrix through epoxy activation. The optimized resin pore size helps with the increasing in binding capacity of the antibody. The resin is suitable for capturing monoclonal antibodies or Fc fusion proteins from a large amount of cell culture media, as well as polyclonal antibodies from ascites fluid or plasma.

The ligands of AT Protein A Diamond Plus are genetically engineered recombinant protein A fragments. In the upstream construction, the alkali-tolerant amino acids were used to replace the non-alkali amino acids, while protease-sensitive amino acids were removed, to provide ligand with good stability. The ligand fermentation and subsequent purification process use animal-free raw materials. The resin can be cleaned in place with 0.1-0.5M NaOH, which avoids the use of expensive and corrosive cleaning reagents, effectively cutting costs. In addition, compared with AT Protein A Diamond, the new resin enjoys higher binding capacity, as well as lower production cost due to the smaller CV selected in scale-up process.

The resin has the following characteristics:

l High rigidity, low back pressure and fast flow velocity, suitable for large-scale production applications.

l High binding capacity, reduce production costs.

l Good alkaline tolerance, longer service life.

2. Technical characteristics

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.

++Linear flow velocity 100cm / h, column height 20cm, buffer conditions: 20mM PB, 0.15M NaCl, pH7.4.

+++2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information