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Protein A Affinity Resins

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Protein A Affinity Resins

AT Protein A Diamond Plus

AT Protein A Diamond Plus

Product Characteristics

  • High hardness, low back pressure and fast flow velocity, suitable for large-scale production applications.
  • High load capacity can reduce production costs.
  • It is resistant to alkaline cleaning and has a long service life.
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Description
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1. Introduction 

AT Protein A Diamond Plus is a affinity resin made by coupling alkali-resistant Protein A with a high-rigidity agarose base through epoxy activation. he optimized resin pore size is conducive to increasing the binding capacity of the antibody.The resin is suitable for capturing monoclonal antibodies or Fc fusion proteins from a large amount of cell culture fluid, and also suitable for capturing polyclonal antibodies from ascites fluid or plasma.
The ligands of AT Protein A Diamond Plus are genetically engineered recombinant protein A fragments. In the upstream construction, the alkali-tolerant amino acids were used to replace the non-alkali amino acids, and the protease-sensitive amino acids were removed, so that the ligand has good stability. The ligand fermentation and subsequent purification process use animal-free raw materials. The resin can be cleaned in place with 0.1-0.5M NaOH, which avoids the use of expensive and corrosive cleaning reagents, which can effectively save costs. At the same time, compared with AT Protein A Diamond, the loading capacity is higher, and a smaller column volume can be selected during the process scale-up process to reduce the production cost.
The resin has the following characteristics:
High hardness, low back pressure and fast flow velocity, suitable for large-scale production applications.
High load capacity can reduce production costs.
It is resistant to alkaline cleaning and has a long service life.


2. Technical Characteristics

Appearance

White slurry, can be layered

Matrix

High rigidity agarose

Particle size +

40~120μm

Functional group

Recombinant Alkali Tolerant Protein A (~24KD)

Cross-linking method

Epoxy chemistry

Dynamic binding capacity++

≥60mg human IgG/mL packed resin

Chemical stability

Stable in common aqueous buffers: 10mM HAc, 0.1M sodium citrate,6M GuHCl,8M Urea, 30% isopropyl alcohol, 20% ethanol.

Max. pressure

0.5MPa

Pressure flow velocity

~500cm/h,

pH stability

3~12(working) 2~13(CIP) CIP: 0.1~0.5M NaOH

Storage+++

2~8℃, 20% ethanol or 2% benzyl alcohol

Recommended flow velocity

60~300cm/h

 

 

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++Linear flow velocity 100cm / h, column height 20cm, buffer conditions: 20mM PB, 0.15M NaCl, pH7.4
+++2% benzyl alcohol is only used for international transport or special requirements from customer

3. Order information

Product

Code No.

Pack size

AT Protein A Diamond Plus

AA402305

25mL

AA402307

100mL

AA402311

500mL

AA402312

1L

AA402313

5L

AA402314

10L

AA402315

20L

 

Prepacked columns

Code No.

Pack size

EzFast AT Protein A Plus

EA402301

1×1mL

EA402303

1×5mL

EA402351

5×1mL

EA402353

5×5mL

EzScreen AT Protein A Plus

EA03625

1×4.9mL

EA03635

5×4.9mL

EzLoad 16/10 AT Protein A Plus

EA402304

1 pcs

EzLoad 26/10 AT Protein A Plus

EA402306

1 pcs

 

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