Extrem A Diamond is an innovative enhanced alkali-resistant resin independently developed by Bestchrom. The protein A ligand is expressed in E. coli. Compared with previous AT Protein A Diamond Plus, it enjoys higher tolerance towards alkali (can tolerate CIP with 0.5-1.0M NaOH) and higher binding capacity. It can effectively cut cost during scale-up by choosing lower column volume. Besides, It is suitable for capturing monoclonal antibodies or Fc fusion protein from large volume cell culture media, as well as the capture of polyclonal antibodies from ascites or plasma.
The resin has the following characteristics:
l Under the same residence time, it has higher dynamic binding capacity than conventional products.
l The high alkaline tolerance of protein ligands can withstand CIP of 0.5~1.0M NaOH during the cleaning process.
l High binding capacity, reduced production cost and improved production efficiency.
Appearance |
White slurry |
Matrix |
High rigidity agarose |
Average particle size + |
~60μm |
Functional group |
Recombinant Alkali Tolerant Protein A |
Cross-linking method |
Epoxy chemistry |
Dynamic binding capacity ++ |
≥75mg Human IgG/mL packed resin(Residence time 6min) |
Chemical stability |
Stable in common aqueous buffers: 10mM HCl, 0.1Msodium citrate, 6M GuHCl, 8M Urea, 30% Isopropyl alcohol, 20% ethanol. |
Max. pressure |
0.5MPa |
Pressure flow velocity |
≥300cm/h,<2bar,BXK300/500,H=20cm |
pH stability |
3~12(working) 2~14(CIP) |
Storage+++ |
2~8℃, 20% ethanol or 2% benzyl alcohol |
+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++Linear flow velocity 100cm / h, column height 10cm, buffer conditions: 20mM PB, 0.15M NaCl, pH7.4.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Extrem A Diamond |
AA04501 |
25mL |
AA04502 |
100mL |
|
AA04503 |
500mL |
|
AA04504 |
1L |
|
AA04505 |
5L |
|
AA04506 |
10L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast Extrem A Diamond |
EA04521 |
1×1mL |
EA04523 |
1×5mL |
|
EA04531 |
5×1mL |
|
EA04533 |
5×5mL |
|
EzScreen Extrem A Diamond |
EA04525 |
1×4.6mL |
EA04535 |
5×4.6mL |
|
EzSelect Extrem A Diamond |
EA04526 |
1×600μL |
EzLoad 16/10 Extrem A Diamond |
EA04501 |
1 pcs |
EzLoad 26/10 Extrem A Diamond |
EA04511 |
1 pcs |