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Benzamidine Bestarose 4FF is an affinity chromatography resin made by coupling p-amino benzamidine to Bestarose 4FF agarose resin. It is commonly used for the separation and purification of serine proteases or the removal of serine proteases from biological samples. The benzamidines are broad-spectrum inhibitors of serine proteases (such as trypsin, thrombin, urokinase, kallikrein, prokinin releasing enzyme, etc) and can be used as ligands for purifying such substances.
|
Appearance |
White slurry |
|
Matrix |
Cross-linked agarose, 4% |
|
Particle size+ |
45~165μm |
|
Functional group |
P-aminobenzidine |
|
Ligand concentration |
≥12μmol Ligand/mL resin |
|
Dynamic binding capacity |
≥35mg Trypsin/mL packed resin |
|
Max. pressure |
0.3MPa |
|
Pressure flow velocity |
＞150cm/h（0.1MPa BXK50/30, H=25cm, 20℃） |
|
Chemical stability |
Stable in common aqueous buffers: 6M GuHCl,8M Urea, PH =1, 2, 3, 4 hydrochloric acid solution, 0.025M borax solution with pH=8, 9, 10, 11 |
|
pH stability |
2~8(working),1~9(CIP) |
|
Storage++ |
2~8℃,20% ethanol + 50mM NaAc(pH4.0) or 2% benzyl alcohol + 50mM NaAc(pH 4.0) |
|
Recommended flow velocity |
30-300cm/h |
+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
Benzamidine Bestarose 4FF |
AA0291 |
25mL |
|
AA0292 |
100mL |
|
|
AA111311 |
500mL |
|
|
AA0294 |
1L |
|
|
AA0295 |
5L |
|
|
AA111314 |
10L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Benzamidine 4FF |
EA111301 |
1×1mL |
|
EA111303 |
1×5mL |
|
|
EA111351 |
5×1mL |
|
|
EA111353 |
5×5mL |
|
|
EzScreen Benzamidine 4FF |
EA02925 |
1×4.6mL |
|
EA02935 |
5×4.6mL |
|
|
EzLoad 16/10 Benzamidine 4FF |
EA111304 |
1 pcs |
|
EzLoad 26/10 Benzamidine 4FF |
EA111306 |
1 pcs |
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