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Bestarose 4FF(Fast Flow)and Bestarose 6FF are SEC resins made by 4% and 6% agarose, respectively. They are used for separation, purification, or detection and biological macromolecules including polysaccharides, nucleic acids and viruses. This resin is often used as a matrix for affinity, HIC and IEX resins. Bestarose 4FF and Bestarose 6FF are made on Bestarose 4B and Bestarose 6B base matrix via crosslinkers. In addition, its degree of crosslinking is higher than that of Bestarose CL-4B and Bestarose CL-6B, enabling better physical and chemical properties, 121°C autoclaving and fast flow velocity, which is suitable for large-scale separation and purification applications.
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Product |
Bestarose 4FF |
Bestarose 6FF |
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|
Appearance |
White slurry |
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|
Agarose concentration |
4% |
6% |
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|
Separation range |
Linear numerator |
30KD~5000KD |
10KD~1000KD |
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Globular molecule |
60KD~20000KD |
10KD~4000KD |
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Particle size + |
45~165μm |
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Average particle size |
~90μm |
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Pressure flow velocity++ |
200-300cm/h（0.1MPa BXK50/60,H=25cm,20°C,in water） |
≥200cm/h（0.1MPa BXK50/60,H=25cm,20°C,in water） |
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Max. pressure |
0.3MPa(3bar) |
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pH stability |
2~12(working),2~14(CIP) |
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Chemical stability |
Stable in common aqueous buffers: 0.5M NaOH+++,2M NaOH+++,6M GuHCl,8M Urea, 1M acetic acid, 30% acetonitrile |
Stable in common aqueous buffers: 0.5M NaOH+++,2M NaOH+++,1M acetic acid, 30% acetonitrile, 8M Urea, 6M GuHCl |
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Sterilization |
Can tolerate 121℃ autoclaving (20min) |
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Storage++++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
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+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ The pressure flow velocity describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin.
+++0.5M NaOH and 2M NaOH only be used for cleaning.
++++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
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Product |
Cat. No. |
Pack size |
|
Bestarose 4FF |
AG0052 |
100mL |
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AG0053 |
500mL |
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|
AG0054 |
1L |
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|
AG0055 |
5L |
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|
AG0056 |
10L |
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|
AG2157 |
20L |
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|
AG214316 |
40L |
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|
Bestarose 6FF |
AG0062 |
100mL |
|
AG0063 |
500mL |
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|
AG0064 |
1L |
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|
AG0065 |
5L |
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|
AG0066 |
10L |
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|
AG2167 |
20L |
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|
AG215316 |
40L |
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Prepacked columns |
Cat. No. |
Pack size |
|
EzLoad 16/60 Bestarose 4FF |
EG214301 |
1 pcs |
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EzLoad 26/60 Bestarose 4FF |
EG214302 |
1 pcs |
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EzLoad 16/90 Bestarose 4FF |
EG027 |
1 pcs |
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EzLoad 26/90 Bestarose 4FF |
EG00513 |
1 pcs |
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EzLoad 16/60 Bestarose 6FF |
EG215301 |
1 pcs |
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EzLoad 26/60 Bestarose 6FF |
EG215302 |
1 pcs |
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EzLoad 16/90 Bestarose 6FF |
EG028 |
1 pcs |
|
EzLoad 26/90 Bestarose 6FF |
EG00613 |
1 pcs |
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