Butyl Bestarose 4FF-HIC Resins丨Bestchrom

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1. Introduction

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Butyl Bestarose 4FF is formed by coupling butyl chain on agarose base matrix. The High physical and chemical stability of agarose base matrix provides it with good pressure flow velocity performance, making it an ideal choice for large-scale purification process, especially in the capture or intermediate purification stages where a high flow velocity separation process is required. The optimized ligand concentration is suitable for the separation and purification of biological molecules with strong hydrophobicity.

2. Technical characteristics

Appearance	White slurry
Matrix	Highly cross-linked agarose
Functional group	Butyl
Ligand concentration	~ 40μmol Ligand/mL resin
Particle size⁺	45~165μm(the average particle size is 90μm)
Max. pressure	0.3MPa
Pressure flow velocity	≥150cm/h(0.1MPa BXK50/60, H= 25cm, 20℃)
Chemical stability	Stable in common aqueous buffers: 1M NaOH⁺⁺,1M HAc⁺⁺,10mM NaOH,6M GuHCl,8M Urea,30% isopropyl alcohol,70% ethanol,20% ethanol,2%benzyl alcohol
pH stability	2~14(CIP),3~13(working)
Operating temperature	Operating temperature: 2~40℃, do not freeze
Storage⁺⁺⁺	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	90~150cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++1M NaOH and 1M HAc only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
Butyl Bestarose 4FF	AH0011	25mL
	AH103307	100mL
	AH103311	500mL
	AH0013	1L
	AH0014	5L
	AH0015	10L
	AH103315	20L
	AH103316	40L

Prepacked columns	Cat. No.	Pack size
EzFast Butyl 4FF	EH00121	1×1mL
	EH002	5×1mL
	EH103303	1×5mL
	EH003	5×5mL
EzScreen Butyl 4FF	EH00125	1×4.6mL
EzScreen Butyl 4FF	EH00135	5×4.6mL
EzLoad 16/10 Butyl 4FF	EH004	1 pcs
EzLoad 26/10 Butyl 4FF	EH005	1 pcs