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Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
The base matrix of Butyl Bestarose HP resin is highly cross-linked agarose, which retains the excellent hydrophilicity and pore structure of natural polysaccharide compounds as well as the good compatibility with biological macromolecules. The average particle size of Butyl Bestarose HP is 34μm, providing a higher resolution than Butyl Bestarose FF. The surface contains hydrophobic Butyl ligands, which is particularly suitable for the polishing of recombinant proteins, antibodies, vaccines, VLP particles and other highly hydrophobic biological molecules.
|
Appearance |
White slurry |
|
Matrix |
Highly cross-linked agarose |
|
Functional group |
Butyl |
|
Ligand concentration |
~50μmol Ligand/mL resin |
|
Dynamic binding capacity |
38mg β- Lactoglobulin /mL packed resin |
|
Particle size+ |
24~44μm(the average particle size is 34μm) |
|
Max. pressure |
0.3MPa |
|
Pressure flow velocity |
≥200cm/h(0.1MPa BXK50/30, H=15cm, 25℃) |
|
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++,1M HAc++,6M GuHCl,30% isopropyl alcohol,70% ethanol |
|
pH stability |
2~14(CIP),3~12(working) |
|
Operating temperature |
Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ autoclaving (20min) |
|
Storage+++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
|
Recommended flow velocity |
60~120cm/h |
+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++1M NaOH and 1M HAc only be used for cleaning.
+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
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Product |
Cat. No. |
Pack size |
|
Butyl Bestarose HP |
AH0071 |
25mL |
|
AH203107 |
100mL |
|
|
AH203111 |
500mL |
|
|
AH0073 |
1L |
|
|
AH0074 |
5L |
|
|
AH203114 |
10L |
|
|
AH203115 |
20L |
|
|
AH203116 |
40L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Butyl HP |
EH00721 |
1×1mL |
|
EH103151 |
5×1mL |
|
|
EH103103 |
1×5mL |
|
|
EH103153 |
5×5mL |
|
|
EzScreen Butyl HP |
EH00725 |
1×4.6mL |
|
EH00735 |
5×4.6mL |
|
|
EzLoad 16/10 Butyl HP |
EH00701 |
1 pcs |
|
EzLoad 26/10 Butyl HP |
EH00711 |
1 pcs |
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