Butyl-S Bestarose FF-HIC Resins丨Bestchrom

Butyl-S Bestarose FF

Product Characteristics

HIC resin using Butyl-S as its ligands
Ligands with weak hydrophobicity, suitable for the capture and intermediate purification of macro-biomolecules with strong hydrophobicity
Agarose matrix with bead size of 90μm, good non-specific adsorption and pressure/flow characteristic.

Diamond Butyl Mustang

Butyl Bestarose 4FF

Description

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1. Introduction

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Butyl-S Bestarose FF is formed by coupling butyl chain on agarose base matrix. The optimized ligand concentration is suitable for the separation and purification of biological molecules with strong hydrophobicity.

2. Technical characteristics

Appearance	White slurry
Matrix	Highly cross-linked agarose
Functional group	Butylthio
Ligand concentration	~ 10μmol Ligand/mL resin
Particle size⁺	45~165μm(the average particle size is 90μm)
Max. pressure	0.3MPa
Pressure flow velocity	250~400cm/h(0.1MPa BXK50/60, H =25cm, 20℃)
Chemical stability	Stable in common aqueous buffers: 20% ethanol ,6M GuHCl,0.5% SDS,8M Urea
pH stability	2~14(CIP),3~13(working)
Operating temperature	Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ autoclaving (20min)
Storage⁺⁺	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	150~300cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
Butyl-S Bestarose FF	AH0061	25mL
	AH204307	100mL
	AH204311	500mL
	AH0063	1L
	AH0064	5L
	AH0065	10L
	AH204315	20L
	AH204316	40L

Prepacked columns	Cat. No.	Pack size
EzFast Butyl-S FF	EH00621	1×1mL
	EH027	5×1mL
	EH204303	1×5mL
	EH028	5×5mL
EzScreen Butyl-S FF	EH00625	1×4.6mL
EzScreen Butyl-S FF	EH00635	5×4.6mL
EzLoad 16/10 Butyl-S FF	EH029	1 pcs
EzLoad 26/10 Butyl-S FF	EH030	1 pcs