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Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
CM Bestarose FF is a weak cation exchanger formed by coupling carboxymethyl onto high flow velocity agarose matrix.
|
Appearance |
White slurry |
|
Matrix |
Cross-linked agarose, 6% |
|
Functional group |
Carboxy methyl |
|
Particle size+ |
45~165μm |
|
Ionic capacity |
90~130μmol H+/mL packed resin |
|
Max. pressure |
0.3MPa |
|
Pressure flow velocity++ |
≥300cm/h（0.1MPa BXK50/30,H=15cm ,20℃,0.1M NaCl） |
|
Chemical Stability |
Stable in common aqueous buffers: 1M NaOH+++,1M HAc+++,6M GuHCl,8M Urea, 70% ethanol ,30% isopropyl alcohol, 20% ethanol, 2%benzyl alcohol |
|
pH stability |
2~14(CIP),4~13 (Working) |
|
Operating temperature |
Operating temperature:2~40℃, do not freeze |
|
Storage++++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
|
Recommend flow velocity |
60~300cm/h |
|
Avoid |
Avoid contact with oxidizing agents, cationic detergents. Avoid long time exposures (1 week, 20℃)to pH <4. |
+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ The pressure flow velocity describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin.
+++1M NaOH and 1M HAc only be used for cleaning.
++++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
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Product |
Cat. No. |
Pack size |
|
CM Bestarose FF |
AI0041 |
25mL |
|
AI0042 |
100mL |
|
|
AI0043 |
500mL |
|
|
AI0044 |
1L |
|
|
AI0045 |
5L |
|
|
AI0046 |
10L |
|
|
AI0047 |
20L |
|
|
AI201316 |
40L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast CM FF |
EI00421 |
1×1mL |
|
EI016 |
5×1mL |
|
|
EI201303 |
1×5mL |
|
|
EI017 |
5×5mL |
|
|
EzScreen CM FF |
EI00425 |
1×4.6mL |
|
EI00435 |
5×4.6mL |
|
|
EzLoad 16/10 CM FF |
EI019 |
1 pcs |
|
EzLoad 26/10 CM FF |
EI020 |
1 pcs |
|
EzLoad 16/20 CM FF（Double stigma head） |
EI051 |
1 pcs |
|
EzLoad 10/10 CM FF（Double stigma head） |
EI054 |
1 pcs |
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