Ion exchange (IEX) chromatography is a very effective method for separation and purification of biological molecules. The method mainly relies on the interaction between positive and negative charges and uses the nature and difference of the charges carried by different biomolecules under specific conditions to separate. It has the characteristics of high load capacity, good resolution, controllable conditions and easy amplification. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
The ion exchange resin is composed of three parts: (1) Cross-linked mesh base frame, which is porous, hydrophilic and chemically stable. (2)The functional group fixed on the base frame, which is a charged group, determines the nature of the ion exchange chromatography resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
CM Bestdex C-25 is a weak cation exchange resin formed by coupling carboxymethyl groups on cross-linked dextran microspheres. It retains the excellent hydrophilicity and pore structure of natural polysaccharide compounds, good compatibility with biologically active molecules, and very low non-specific adsorption. CM Bestdex C-25 is based on dextran matrix, which has a very high capacity for small molecular substances and is very suitable for the separation and purification of antibiotics, natural products and other substances. Due to the large variation of the column bed volume of the chromatography resin in different buffers, it is not suitable to be installed as a fixed bed chromatography column.
Appearance |
White to slight yellow powdery solid |
Matrix |
Cross-linked dextran |
Functional group |
Carboxymethyl |
Dry powder size |
40~120μm |
Swelling coefficient |
7mL/g dry powder(PBS) |
Ionic capacity |
4.0~5.0mmol H+/g dry powder |
Max. pressure |
0.3MPa |
Working pH range |
6~10 |
pH stability |
2~13(CIP),2~12 (working) |
Chemical Stability |
Stable in common aqueous buffers: 6M GuHCl,8M Urea, 70% ethanol , 30% isopropyl alcohol |
Operating temperature |
Operating temperature:2~40℃, do not freeze, Can tolerate 121℃ autoclaving (add 50mM PB pH 7) |
Recommended flow velocity |
60~150cm/h |
Product |
Cat. No. |
Pack size |
CM Bestdex C-25 |
AI0261 |
25g |
AI0262 |
100g |
|
AI0263 |
500g |
|
AI0264 |
1kg |
|
AI0265 |
5kg |
|
AI0267 |
10kg |
|
AI0268 |
20kg |
|
AI0266 |
25kg |