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Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determining the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
DEAE Bestarose HP (high performance) is a weak cation exchange resin formed by coupling diethylaminoethyl functional groups onto high flow velocity agarose matrix.
|
Appearance |
White slurry |
|
Matrix |
Cross-linked agarose |
|
Functional Group |
Diethylaminoethyl |
|
Particle size+ |
24~44μm (the average particle size is 34μm) |
|
Ionic capacity |
110-160μmol/ mL packed resin |
|
Max. pressure |
0.3MPa |
|
Pressure flow velocity |
≥200cm/h (0.1MPa, BXK50/30, H=15cm, 25℃) |
|
Working pH range |
2~9 |
|
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++,6M GuHCl,8M Urea, 70% ethanol ,30% isopropyl alcohol |
|
pH Stability |
2~14(CIP), 2~12 (Working) |
|
Operating temperature |
Operating temperature:2~40℃, do not freeze. |
|
Storage+++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
|
Recommended flow velocity |
60~150cm/h |
|
Avoid |
Avoid contact with oxidizing agents, cationic detergents. Avoid long time exposures (1 week, 20℃) to pH <4. |
+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++1M NaOH only be used for cleaning.
+++ 2% benzyl alcohol is only used for international transport or special requirements from customer
|
Product |
Cat. No. |
Pack size |
|
DEAE Bestarose HP |
AI0301 |
25mL |
|
AI0302 |
100mL |
|
|
AI0303 |
500mL |
|
|
AI0304 |
1L |
|
|
AI0305 |
5L |
|
|
AI0306 |
10L |
|
|
AI0307 |
20L |
|
|
AI0308 |
40L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast DEAE HP |
EI03021 |
1×1mL |
|
EI03031 |
5×1mL |
|
|
EI03023 |
1×5mL |
|
|
EI03033 |
5×5mL |
|
|
EzScreen DEAE HP |
EI03025 |
1×4.6mL |
|
EI03035 |
5×4.6mL |
|
|
EzLoad 16/10 DEAE HP |
EI03001 |
1 pcs |
|
EzLoad 26/10 DEAE HP |
EI03011 |
1 pcs |
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