Diamond Butyl-HIC Resins丨Bestchrom

Diamond Butyl

Product Characteristics

HIC resin using Butyl as its ligands
Suitable for the capture and intermediate purification of proteins, which require HIC resins with low and intermediate hydrophobicity
High rigid agarose matrix, enjoys high flow rate and low back pressure
Different binding capacity and selectivity compared with Butyl Bestarose FF resin

Butyl Bestarose HP

Butyl-S Bestarose FF

Description

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1. Introduction

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Diamond Butyl is formed by coupling weakly hydrophobic aliphatic butyl groups on high-rigidity agarose matrix. It has low back pressure and fast flow velocity, which is suitable for large-scale purification of highly hydrophobic biomolecules.

2. Technical characteristics

Appearance	White slurry
Matrix	Highly cross-linked rigid agarose
Functional group	Butyl
Ligand concentration	~53μmol Ligand/mL resin
Dynamic binding capacity	~27mg BSA/mL packed resin
Particle size⁺	40~120μm
Max. pressure	0.5MPa
Pressure flow velocity	≥1200cm/h(0.5MPa BXK 100/500, H=20cm, 20℃)
Chemical stability	Stable in common aqueous buffers: 1M NaOH++,1M HAc++,6M GuHCl,30% isopropyl alcohol,70% ethanol
pH stability	2~14(CIP),3~13 (working)
Operating temperature	Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ autoclaving (20min)
Storage+++	2~30℃,20% ethanol or 2% benzyl alcohol
Recommended flow velocity	150~350cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.

++1M NaOH and 1M HAc only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
Diamond Butyl	AH0111	25mL
	AH0112	100mL
	AH303311	500mL
	AH0113	1L
	AH303313	5L
	AH303314	10L
	AH303315	20L
	AH303316	40L

Prepacked columns	Cat. No.	Pack size
EzFast Diamond Butyl	EH01121	1×1mL
	EH303351	5×1mL
	EH303303	1×5mL
	EH303353	5×5mL
EzScreen Diamond Butyl	EH01125	1×4.6mL
EzScreen Diamond Butyl	EH01135	5×4.6mL
EzLoad 16/10 Diamond Butyl	EH303204	1 pcs
EzLoad 26/10Diamond Butyl	EH303206	1 pcs