Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
Diamond Butyl Mustang is based on fine-grained high-rigidity agarose with weak hydrophobic aliphatic butyl groups. It has high resolution and low back pressure, and is suitable for the intermediate and fine purification stages of protein downstream purification.
Appearance |
White slurry |
Matrix |
Highly cross-linked rigid agarose |
Functional group |
Butyl |
Average particle size |
36~44μm |
Hydrophobicity |
The retention time of α - Chymotrypsinogen is 52-58min |
Dynamic binding capacity |
~37mg BSA/mL packed resin |
Max. pressure |
0.5MPa |
Pressure flow velocity |
≥450cm/h(0.5MPa BXK 100/500, H=20cm, 20℃) |
Chemical stability |
Stable in common aqueous buffers: 1M NaOH+,1M HAc+,6M GuHCl,30% isopropyl alcohol,70% ethanol |
pH stability |
2~14(CIP),3~13(working) |
Operating temperature |
Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ autoclaving (20min) |
Storage++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
Recommended flow velocity |
90~180cm/h |
+1M NaOH and 1M HAc only be used for cleaning.
++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Diamond Butyl Mustang |
AH303205 |
25mL |
AH303207 |
100mL |
|
AH303211 |
500mL |
|
AH303212 |
1L |
|
AH303213 |
5L |
|
AH303214 |
10L |
|
AH303215 |
20L |
|
AH303216 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast Butyl Mustang |
EH01621 |
1×1mL |
EH303251 |
5×1mL |
|
EH303203 |
1×5mL |
|
EH303253 |
5×5mL |
|
EzScreen Butyl Mustang |
EH01625 |
1×4.6mL |
EH01635 |
5×4.6mL |
|
EzLoad 16/10 Butyl Mustang |
EH01601 |
1 pcs |
EzLoad 26/10 Butyl Mustang |
EH01611 |
1 pcs |