Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. Traditional agarose molecules are modified to make them have better mechanical properties, forming high rigidity agarose, also known as Diamond matrix. (2)Functional groups fixed to the matrix, which are electrically charged groups, determining the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Diamond CM is a kind of weak cation exchange resin formed by coupling carboxymethyl groups on high rigid agarose microspheres, which has stronger pressure resistance and higher binding capacity than CM Bestarose FF.
Appearance |
White slurry |
Matrix |
Highly-rigidity agarose with long dextran chain |
Functional group |
Carboxymethyl |
Particle size+ |
45~165μm |
Ionic capacity |
90~130μmol H+/mL packed resin |
Dynamic binding capacity |
~50 mg BSA/ mL packed resin |
Max. pressure |
0.5MPa |
Pressure flow velocity |
≥1500cm/h (0.5MPa BXK 100/500, H=20cm, 20℃) |
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++,1M HAC++, 6M GuHCl,8M Urea, 30% isopropyl alcohol, 70% ethanol, 20% ethanol, 2% benzyl alcohol |
pH stability |
4~13 (working),2~14(CIP) |
Operating temperature |
Operating temperature:2~40℃, do not freeze |
Storage+++ |
2~30℃,20%ethanol or 2% benzyl alcohol |
Recommended flow velocity |
90~500cm/h |
Avoid |
Avoid contact with oxidizing agents, cationic detergents. Avoid long time exposures (1 week, 20℃)to pH <4. |
+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++1M NaOH and 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Diamond CM |
AI311305 |
25mL |
AI311307 |
100mL |
|
AI311311 |
500mL |
|
AI311312 |
1L |
|
AI311313 |
5L |
|
AI311314 |
10L |
|
AI311315 |
20L |
|
AI311316 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast Diamond CM |
EI03621 |
1×1mL |
EI03631 |
5×1mL |
|
EI03623 |
1×5mL |
|
EI03633 |
5×5mL |
|
EzScreen Diamond CM |
EI03625 |
1×4.6mL |
EI03635 |
5×4.6mL |
|
EzLoad 16/10 Diamond CM |
EI03601 |
1 pcs |
EzLoad 26/10 Diamond CM |
EI03611 |
1 pcs |