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Weak Cation Exchange Resins

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Ion Exchange Resins
Weak Cation Exchange Resins

Diamond CM

Diamond CM

Product Characteristics

  • Weak CEX resin based on high rigid agarose matrix
  • High rigid, low back pressure, suitable for rapid processing of large sample volume at high flow rate
  • Good mechanical and chemical stability, low non-specific adsorption
  • Widely applicable in the purification of large-volume recombinant proteins in bio-pharmaceuticals and bio-engineering.
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Description
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1. Introduction

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. Traditional agarose molecules are modified to make them have better mechanical properties, forming high rigidity agarose, also known as Diamond matrix. (2)Functional groups fixed to the matrix, which are electrically charged groups, determining the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

Diamond CM is a kind of weak cation exchange resin formed by coupling carboxymethyl groups on high rigid agarose microspheres, which has stronger pressure resistance and higher binding capacity than CM Bestarose FF.

 

2. Technical characteristics

Appearance

White slurry

Matrix

Highly-rigidity agarose with long dextran chain

Functional group

Carboxymethyl

Particle size+

45~165μm

Ionic capacity

90~130μmol H+/mL packed resin

Dynamic binding capacity

~50 mg BSA/ mL packed resin

Max. pressure

0.5MPa

Pressure flow velocity

≥1500cm/h (0.5MPa BXK 100/500, H=20cm, 20℃)

Chemical stability

Stable in common aqueous buffers: 1M NaOH++,1M HAC++, 6M GuHCl,8M Urea, 30% isopropyl alcohol, 70% ethanol, 20% ethanol, 2% benzyl alcohol

pH stability

4~13 (working),2~14(CIP)

Operating temperature

Operating temperature:2~40℃, do not freeze

Storage+++

2~30℃,20%ethanol or 2% benzyl alcohol

Recommended flow velocity

90~500cm/h

Avoid

Avoid contact with oxidizing agents, cationic detergents.

Avoid long time exposures (1 week, 20℃)to pH <4.

 

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++1M NaOH and 1M HAc only be used for cleaning.

+++2% benzyl alcohol is only used for international transport or special requirements from customer.

 

3. Order information

Product

Cat. No.

Pack size

Diamond CM

AI311305

25mL

AI311307

100mL

AI311311

500mL

AI311312

1L

AI311313

5L

AI311314

10L

AI311315

20L

AI311316

40L

 

Prepacked columns

Cat. No.

Pack size

EzFast Diamond CM

EI03621

1×1mL

EI03631

5×1mL

EI03623

1×5mL

EI03633

5×5mL

EzScreen Diamond CM  

EI03625

1×4.6mL

EI03635

5×4.6mL

EzLoad 16/10 Diamond CM

EI03601

1 pcs

EzLoad 26/10 Diamond CM

EI03611

1 pcs

 

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