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Ion exchange chromatography (IEX) is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability, Traditional agarose molecules are modified to have better mechanical properties, which is called high rigid agarose, or Diamond base matrix. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Diamond DEAE is a weak anion exchange resin formed by coupling diethylaminoethyl to high-rigidity agarose microspheres.
|
Appearance |
White slurry, can be layered |
|
Matrix |
Highly-rigidity agarose with long dextran chain |
|
Functional group |
Diethylaminoethyl |
|
Average particle size+ |
~90μm |
|
Ionic capacity |
290~350μmol Cl-/mL packed resin |
|
Dynamic binding capacity |
> 90mg ovalbumin /mL packed resin |
|
Max. pressure |
0.5MPa |
|
Pressure flow velocity |
≥1500cm/h(0.5MPa ,BXK 100/500 ,H=20cm, 20℃) |
|
pH stability |
2~9(working),2~14(CIP) |
|
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++,1M HAC++, 6M GuHCl,8M Urea, 30% isopropyl alcohol, 70% ethanol Avoid contact with oxidizing agents, cationic detergents. |
|
Temperature tolerance |
Working temperature:2~40℃, Can't freeze. |
|
Storage+++ |
2~30℃,20%ethanol or 2% benzyl alcohol |
|
Recommended flow velocity |
90~500cm/h |
+ The average particle size is the cumulative resin particle size of the filler volume distribution.
++1M NaOH and 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
Diamond DEAE |
AI0121 |
25mL |
|
AI0122 |
100mL |
|
|
AI312311 |
500mL |
|
|
AI0123 |
1L |
|
|
AI0124 |
5L |
|
|
AI0125 |
10L |
|
|
AI312315 |
20L |
|
|
AI312316 |
40L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Diamond DEAE |
EI01221 |
1×1mL |
|
EI312351 |
5×1mL |
|
|
EI312303 |
1×5mL |
|
|
EI312353 |
5×5mL |
|
|
EzScreen Diamond DEAE |
EI01225 |
1×4.6mL |
|
EI01235 |
5×4.6mL |
|
|
EzLoad 16/10 Diamond DEAE |
EI312304 |
1 pcs |
|
EzLoad 26/10 Diamond DEAE |
EI312306 |
1 pcs |
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