Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. Traditional agarose molecules are modified to have better mechanical properties, which is called high rigid agarose, or Diamond base matrix. (2)Functional groups fixed to the matrix, which are electrically charged groups, determining the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Diamond DEAE Mustang is a weak anion exchange resin formed by coupling diethylaminoethyl groups on small and high rigidity agarose microspheres. Diamond DEAE has a higher resolution than Diamond DEAE and is suitable for polishing and intermediate stage in the chromatography process.
Appearance |
White slurry |
Matrix |
High rigidity agarose |
Functional group |
Diethylaminoethyl |
Average Particle size+ |
36~44μm |
Ionic capacity |
110-160μmol/mL packed resin |
Max. pressure |
0.5MPa |
Pressure flow velocity |
≥450cm/h (0.5MPa, BXK 100/500, H=20cm, 20℃) |
pH stability |
2~9(working), 2~14 (CIP) |
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++,1M HAC++, 6M GuHCl,8M Urea, 30% isopropyl alcohol, 70% ethanol Avoid contact with oxidizing agents, cationic detergents. |
Operating temperature |
Working temperature:2~40℃, do not freeze. |
Storage+++ |
2~30℃,20% ethanol or 2% benzyl alcohol |
Recommended flow velocity |
100~300cm/h |
+ The average particle size is the cumulative resin particle size of the filler volume distribution.
++1M NaOH and 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Diamond DEAE Mustang |
AI302205 |
25mL |
AI302207 |
100mL |
|
AI302211 |
500mL |
|
AI302212 |
1L |
|
AI302213 |
5L |
|
AI302214 |
10L |
|
AI302215 |
20L |
|
AI302216 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast Diamond DEAE Mustang |
EI03321 |
1×1mL |
EI03331 |
5×1mL |
|
EI03323 |
1×5mL |
|
EI03333 |
5×5mL |
|
EzScreen Diamond DEAE Mustang |
EI03325 |
1×4.6mL |
EI03335 |
5×4.6mL |
|
EzLoad 16/10 Diamond DEAE Mustang |
EI03301 |
1 pcs |
EzLoad 26/10 Diamond DEAE Mustang |
EI03311 |
1 pcs |