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Weak Anion Exchange Resins

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Ion Exchange Resins
Weak Anion Exchange Resins

Diamond DEAE Mustang

Diamond DEAE Mustang

Product Characteristics

  • Weak AEX resin with high resolution
  • High rigid agarose matrix with high resolution, suitable for the intermediate purification and polishing of large sample volume in production.
  • Good mechanical and chemical stability, long working life.
  • Widely applicable in the purification of macro-biomolecules including proteins and nucleic acid in bio-pharmaceuticals and bio-engineering.
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Description
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1. Introduction

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. Traditional agarose molecules are modified to have better mechanical properties, which is called high rigid agarose, or Diamond base matrix. (2)Functional groups fixed to the matrix, which are electrically charged groups, determining the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

Diamond DEAE Mustang is a weak anion exchange resin formed by coupling diethylaminoethyl groups on small and high rigidity agarose microspheres. Diamond DEAE has a higher resolution than Diamond DEAE and is suitable for polishing and intermediate stage in the chromatography process.

 

2. Technical characteristics

Appearance

White slurry

Matrix

High rigidity agarose

Functional group

Diethylaminoethyl

Average Particle size+

36~44μm

Ionic capacity

110-160μmol/mL packed resin

Max. pressure

0.5MPa

Pressure flow velocity

≥450cm/h (0.5MPa, BXK 100/500, H=20cm, 20℃)

pH stability

2~9(working), 2~14 (CIP)

Chemical stability

Stable in common aqueous buffers: 1M NaOH++,1M HAC++, 6M GuHCl,8M Urea, 30% isopropyl alcohol, 70% ethanol

Avoid contact with oxidizing agents, cationic detergents.

Operating temperature

Working temperature:2~40℃, do not freeze.

Storage+++

2~30℃,20% ethanol or 2% benzyl alcohol

Recommended flow velocity

100~300cm/h

 

+ The average particle size is the cumulative resin particle size of the filler volume distribution.

++1M NaOH and 1M HAc only be used for cleaning.

+++2% benzyl alcohol is only used for international transport or special requirements from customer.

 

3. Order information

Product

Cat. No.

Pack size

Diamond DEAE Mustang

AI302205

25mL

AI302207

100mL

AI302211

500mL

AI302212

1L

AI302213

5L

AI302214

10L

AI302215

20L

AI302216

40L

 

Prepacked columns

Cat. No.

Pack size

EzFast Diamond DEAE Mustang

EI03321

1×1mL

EI03331

5×1mL

EI03323

1×5mL

EI03333

5×5mL

EzScreen Diamond DEAE Mustang

EI03325

1×4.6mL

EI03335

5×4.6mL

EzLoad 16/10 Diamond DEAE Mustang

EI03301

1 pcs

EzLoad 26/10 Diamond DEAE Mustang

EI03311

1 pcs

 

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